Matrix Metalloproteinase 7 And 13 In The Human Intervertebral Disc Expression Of Different Structures And The Relationship Between Disc Degeneration

Degenerative disc disease is the orthopedic clinical common disease, including cervical spondylosis, lumbar disc herniation and the lumbar spinal stenosis. Immune inflammatory response, nerves and blood vessels stimulated and oppressed by the nucleoplasty cause the appropriate symptoms and signs troubled patients and surgeon. Domestic and foreign clinical MRI imaging data shows that in the lower age groups there are varying degrees of disc degeneration, but in terms of clinical data analysis, the incidence of such diseases, had large individual differences. In present the pathogenesis of these diseases are not well described, the cause of preventive treatment for little elaboration. Although clinical diagnostic methods, surgical methods in continuous improvement to the treatment of intervertebral disc disease, there are still many problems exist such as: poor efficacy of conservative treatment, surgery complications and so on. These prompted researchers to study disc degeneration disease pathogenesis for more in-depth research. The disc is the largest no blood supply of the human body structure, characterized by degeneration of proteoglycan content decreased, water reduction, collagen fibers content changing. Recent studies show the role of biomechanics is secondary; emphasize biochemics process in the role of disc degeneration. But the start and control of this biochemical process are not clear. Changes in extracellular matrix may be a direct result of disc degeneration. Matrix metalloproteinase plays an important role in intervertebral disc degeneration biochemical process of initiation and regulation, while MMPs-related genes also let people to understand the role of MMPs in the disc degeneration.MMPs are involved in ECM degradation protease family, current research in the disc ECM have more MMPs, including MMP-1, 2,3,7,9,13 and so on. MMP-7, also known as matrilysin, is the smallest MMPs family of independent elements and a more important member, MMP-7 in the extracellular matrix and basement membrane degradation plays an important role. MMP-13 from chondrocytes, not only degradeâ…¡collagen degradation, but also degrade aggrecan, resulting in substrate degradation played a important role. MMP-13 can be increased the activity by a variety of cytokines of the body, including MMP-2, MMP-3 and other MMPs. As an essential component of ECM degradation protease, MMPs play a very important role in disc degeneration. As an important structure of disc sustain way, cartilage endplate change and the expression of cartilage endplate MMPs research is very necessary. By studying the different degrees of degenerative disc MMP-7 and MMP-13 in the cartilage endplate, nucleus pulposus, annulus fibrosus, learning the expression of different structure and distribution, to understand different disc structure of cells, ECM degeneration process in its role of MMP-7 and MMP-13 in the cervical and lumbar disc degeneration.Method:Strictly screening out clinical case of cervical spondylosis, lumbar disc herniation, spinal injury patients in the short term as varying degrees of human intervertebral disc degeneration in patients with a total of 32 patients and divided them into 3 group: disc prominent group, disc prolapse group and the trauma group. All patients had X ray, MRI or other clinical examinations, using the VAS assessed the pain of patients. Cut anulus fibrosus, nucleus pulposus, cartilage endplate, respectively, by surgical treatment. With 4% paraformaldehyde for about 24 hours underwent dehydration, embedding, slicing up and other operations. By HE staining, immunohistochemistry, in situ hybridization detection of disc MMP-7, MMP-13 expression and the content changes in the ECM. Detected disc and all parts of I-type II collagen content by immunohistochemistry, combined with degenerative disc cases of clinical data, studied statistical analysis of experimental results.Results: (1)MMP-7 and MMP-13 expression were high in cartilage endplate and nucleus pulposus of disc prominent group, semi-quantitative immunohistochemical analysis of MMP-7 in a prominent group of cartilage endplate above the prolapse group (p <0.05); positive cells per unit area by immunohistochemical expression of MMP-7 of prominent group cartilage endplate above the prolapse group (p <0.05). (2) MMP-7 expression were high in nucleus pulposus of disc prolapse group, semi-quantitative immunohistochemical analysis of MMP-7 in the prolapse group were higher than prominent group of nucleus pulposus (p <0.05); semi-quantitative immunohistochemical analysis of MMP-13 in the prolapse group cartilage endplate above prominent group (p <0.05); immunohistochemical positive cells per unit area MMP-7 and MMP-13 in prominent group nucleus pulposus were higher than prolapsed group (p <0.05); immunohistochemical expression of positive cells per unit area MMP-7 prolapse group cartilage endplate above the prominent group. (3) Immunohistochemical analysis of prominent groups and prolapse group of three structures MMP-7 and MMP-13 were higher than the trauma group. (4)Positive cells per unit area in situ hybridization, the expression of MMP-13 prominent group cartilage endplate above the prolapse group.Conclusion:The results suggest that: (1) MMP-7 and MMP-13 in the disc degeneration process are highly expressed, and associated with the basic degeneration. (2) The expression of MMP-7, MMP-13 in different degeneration and different disc structure are inconsistent. In early degeneration the MMP-7 expression in the cartilage endplate is high, in middle and late degeneration the MMP-13 expression of intervertebral disc cartilage is high, showing cartilage endplate cells may participate in the process of disc degeneration play different roles in different times.