Effects Of Combinations Of Crude Drug And Components From Herbal Pair Of Oldenlandia Diffusa Roxb And Scutellaria Barbata D.Don On Their Antioxidant Activities

Oldenlandia diffusa Roxb (Hedyotis diffusa Willd) is a well known folk medicine in Southern China and has been used for the treatment of hepatitis, tonsillitis, sore throat, appendicitis, urethral infection and malignant tumors of the liver, lung and stomach. Scutellaria barbata D. Don is a wild perennial plant endemic to southern China, which has been widely used for medicinal purposes, such as inflammation, hepatitis, osteomyelitis, gynecological, lung, and rectal tumors in traditional Chinese medicine. These two plants have been frequently used together, named'Herb Pair'in traditional Chinese medicine, to achieve mutual enhancement or mutual assistance for the treatment of cancer and tumor in the clinical practice. It is indicated that the Herb Pair of these two plants may contain some new chemical components that result in the enhancement of antitumor activities.This article investigate the antioxidant activities and free radical scavenging abilities of Oldenlandia diffusa Roxb and Scutellaria barbata D. Don from combinations of crude drugs and components aspects, in order to find if combinations had an effect on antioxidants of extract, in search of the combination formulas and features, and also provide scientific theory basis for clinical.In the first chapter, the progress in the study of the effects of free radicals on man, the main active components in Chinese medicine herbs, research on herbal pair, chemical elements, pharmacology effect of Hedyotis diffusa Willd, Scutellaria barbata D.Don and clinical application of their Herb Pair in recent years was summarized, to provide a reference for further studies in these fields.In the second chapter, aqueous, hydroalcoholic and organic extracts of three fixed-rations of crude drugs were prepared, and different hydroalcoholic extracts of Oldenlandia diffusa Roxb and Scutellaria barbata D. Don were prepared. Total flavonoids and total phenolics content of extracts were also determined by ultraviolet spectrophotometry and Folin-Ciocalteu method.In the third chapter, the antioxidant and free radical scavenging activities of aqueous, hydroalcoholic and organic extracts of crude drugs were evaluated. The results showed that the antioxidant activities and total flavonoids and phenolics were different related to the extract solvent, and hydroalcoholic extracts revealed higher antioxidant activities and higher content of components, suggesting that hydroalcoholic can extract active compounds from crude drugs.In the fourth chapter, the effects of different combinations on antioxidant capacities of hydroalcoholic extracts of crude drugs were studied, and the coefficients of correlations between antioxidant and total flavonoids were calculated by SPSS. The results indicated that the DPPH,·OH radical scavenging activity and chelating efficacy were affected by various combinations. The antioxidant activities of combination of 1:1 were:DPPH>·OH radical scavenging>chelating efficacy> reducing power, suggesting that extracts revealed stronger radical scavenging activities when drugs mixed with equal proportions. The antioxidant activities of 1:2 and 2:1 were:chelating efficacy>free radical scavenging activities> reducing power, both of them showing stronge chelating efficacy, indicating that it was easy to extract active compounds when drugs mixed with unequal proportions,. At the same time, 1:2 revealed strong free radical scavenging activities, which may be correlated with total flavonoids of extracts. In a word,1:2 was the strong antioxdiant combination which has higher chelating efficacy,·OH,DPPH radical scavenging activities and reducing power, and highest total flavonoids.In the fifth chapter, the interactions of antioxidant activities which influenced by various combination components between flavonoids of Oldenlandia diffusa Roxb and Scutellaria barbata D.Don were studied by isobolographic analysis, the compounds of samples were characterized by RP-HPLC. The results showed that 1:1,2:1,1:2,3:1 and 1:3 formulas revealed synergistic interaction in DPPH radical scavenging and total antioxidant test models, while additive in reducing power model. Various formulas did not change the main compounds of samples, but changed the content of main compounds and antioxidant of samples.