| Objective: In this study, the establishment of hydroxyurea caused by anovulation rat models of astragalus on the body symptoms, serum sex hormone levels, ovarian morphology,ovarian and uterine index index, serum AMH levels to explore and promote Astragalus in anovulatory rats possible mechanism of ovulation, ovulation disorder treatment for the astragalus to provide a scientific basis.METHODS: Hydroxyurea administered method unmated adult female SD rats modeling, observation of vaginal smears, no estrous cycle were included in the experiment. Successful model rats were randomly divided into model group, clomiphene citrate, astragalus high, medium and low dose group, combined with no modeling of the normal group, a total of 6 groups, drug intervention 28 days, rats were killed at diestrus, blood and serum sex hormone, test strip wet weight of ovary and uterus, ovary conventional light microscope specimen preparation.Result1 A generalNormal rats, active, responsive to external stimulation, skin Mao Runze, eating, drinking normal. See the model rats of different degrees fatigue, hair loose, dull, loose stools bad, less activity and body weight has not increased or even reduced symptoms of both kidney and spleen. After drug treatment, the high dose group Astragalus symptoms were improved, which significantly improve the high school group, no obvious improvement clomiphene group.Model group was significantly lower body weight group (P<0.01), drug after the growth rate of rats increased body weight, which Astragalus high, medium and low dose group and model group, clomiphene citrate group were significantly different (P<0.01), and the normal group showed no significant (P> 0.05); clomiphene group body weight increase and the model was no significant difference (P> 0.05). Note the dose of astragalus can improve symptoms of rats with general weakness, more effective than clomiphene citrate group.2 serum FSH, LH, E2 DeterminationModel the level of serum E2 was significantly lower than normal (P<0.05), and clomiphene citrate treatment by Astragalus, Astragalus low dose E2 levels between the groups was not statistically significant (P>0.05), other treatment Serum E2 was significantly increased compared with the model (P<0.05), the serum FSH, LH levels were not significantly different (P> 0.05). Description Astragalus high dose group and the clomiphene citrate group can significantly improve the model of serum E2 levels, thereby improving the reproductive endocrine disorder of the environment in which a large dose of Astragalus significant effect.3 ovarian morphologyNormal rats microscope, well developed at all levels of follicle, mature follicle and secondary follicle and more mature follicles were seen in oocyte and corona radiata, granulosa cells in order. Luteal more; model rats seen in ovarian follicles, but the growth of small follicles, almost no mature follicles, atretic follicles increased; Astragalus high dose group, clomiphene group shows different stages of follicular development and corpus luteum, follicle and secondary follicle the number of near-normal number of groups; Astragalus low dose group and no significant difference between the model group; astragalus visible growth of follicles in dose group than in model group increased to increase the secondary follicle, mature follicle than the high dose group and the clomiphene Astragalus group less. Help with clomiphene citrate group, astragalus treatment group improved in different degrees of ovarian pathology, and promote follicular development and maturation, and showed significant dose-effect relationship, to the best effect of Astragalus high dose group.4 ovarian and uterine indexOvarian index model group was significantly lower than group (P <0.01), Astragalus low dose group compared with the model of ovarian index was no significant difference (P>0.05), the other treatment groups significantly increased ovarian index, with the model group statistically significant difference compared (P<0.05, P<0.01), Astragalus high dose group, clomiphene citrate group compared with the normal ovarian index was no significant difference (P>0.05). Uterine index model group was significantly lower than group (P <0.05), high-dose group and the clomiphene Astragalus group could significantly increase the rat's uterus index (P<0.05), Astragalus low and medium dose group and model group than the difference was not statistically significant (P>0.01). Illustrates the AM can significantly increase the model rat ovarian, uterine weight, the mechanism may be because there are estrogen effect, may be or by increasing the level of serum E2, or directly on the uterus and ovaries, thereby effectively protecting the body's genitals to improve their reproductive function.5 anti-Mullerian hormoneThe levels of serum AMH level was significantly lower than normal group (P<0.05), only the high dose group Astragalus can significantly increase the level of serum AMH, and the normal group no significant difference (P>0.05), and the clomiphene citrate group, the low dose group Astragalus were significantly different (P<0.05), shows high doses of astragalus can significantly improve the model of serum AMH levels, improve ovarian function, ovarian reserve capacity to improve, better than clomiphene citrate.Conclusion:SD rats on the formation of kidney hydroxyurea anovulation modeling animal model for treatment of1 hydroxyurea modeling can successfully anovulation caused kidney rat model, the disappearance of estrous cycle, mature follicle and secondary follicles decreased significantly.2 astragalus may improve symptoms of anovulation in rats kidney to high dose Astragalus best, much better than clomiphene citrate.3 Astragalus may increase significantly rat kidney ovarian, uterine wet weight, increased serum E2 levels, improve pathological ovary, so as to promote follicle growth and maturation. Especially in high dose group was the best, which showed obvious dose-effect relationship.4 High doses of astragalus can increase the level of serum AMH, suggesting that early intervention by anovulation in rats increased ovarian reserve capacity, restoration of ovarian function. |
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