Preparation Of Corn Bran Dietary Fiber By Enzyme Hydrolysis And Its Hypolipidemic Mechanisms

In the paper, the corn straw was used as raw material to extract dietary fiber. The conditions of prepartion high-qulity fiber by cellulase were extensively studied, and the monosaccharide compositions and properties of different dietary fiber samples were measured and analyzed.The response surface analysis methodology (RSM) was used to optimize cellulase assisted extraction conditions in the extraction processes of SDF. On the.Base of the single- factor experiments, four independent variables, extracting time, water- solid ratio ,pH and amount of enzyme , were selected as casual factors during extraction.The experiments were arranged according to Box- Behnken central composite experiment design. Response surface analysis method was determined the effects of central composite experiment design. Response surface analysis method was applied to determine the effect of casual factors on the yield of SDF.The opimal experimental conditions for high-quality dietary fiber from wheat corn by cellulase were the ratio of wheat corn to water was 1:9.2,cellulase addition was 23.1U/g,pH was 4.84, extration time was 2.4h and the rate of DF was 10.6%.Water holding capacity, oil absorption capacity, swelling capacity, soluble matter content, cation exchange capacity, nitrite adsorption capacity, cholesterol absorption capacity, viscosity and particle size distribution of different of differernt dietary fiber samples were also analyed.At last, research on the function of dietary fiber were carried on the effect of soluble dietary fiber on reducing blood lipid in mice. The results showed that SDF had great impact on reducing blood lipid whereas the insoluble dietary fiber had little influence.