| In recent years, with the development of economy and the improvement of life quality, not reasonable diet and brings diseases such as obesity, high blood fat, atherosclerosis and rising gradually.Mushrooms of useful bioactive metabolites were found in the treatment of cholesterol disease with a great potential. To explore the bioactive compounds from edible mushrooms for interfering with the development of macrophage-derived foam cells, which is recognized as the hallmark of early atherosclerosis, eight types of mushrooms polysaccharides had been selected to be tested. Consequently, different mushrooms polysaccharides displayed diverse componentprofiles. Of polysaccharides that we tested the P. eryngii polysaccharide had the strongest inhibitory effect on lipid accumulation.Edible and medicinal fungi eryngii has a high nutritional value, polysaccharides and other active ingredients because of their excellent care and treatment function more and more attention has been paid, but the vast majority of research is limited to the the polysaccharide extraction process optimization, large numbers hovering in the low-level duplicated research on the separation of its active ingredient and the study of the mechanisms of metabolic regulation is also relatively small, eryngii in the active component and its biological activity play unclear.The potential biological functions of Pleurotus eryngii have long been described in Chinese traditional medicine. However, little is known about the bioactive components of P. eryngii and their biological functions in vitro and in vivo. Based on our previous studies, we found that in a high-fat-load mouse model, PEPE not only remarkably decreased the lipid levels of total cholesterol, total triglyceride, and low-density lipoprotein cholesterol, but also resulted in an increase in high-density lipoprotein cholesterol. Moreover, histopathological observations indicated that PEPE could effectively prevent excessive lipid formation in liver tissue. In addition, four different fractions (PEPE-1,2,3, and 4) were separated and purified from PEPE using a DEAE-cellulose anion-exchange column combined with Sephadex G-100 column. After analysis by using high performance liquid chorography, all separate fractions displayed a single peak indicating which may include only one component. Notably, PEPE-1 and 2 had the potential properties that remarkably increased the lipid efflux in macrophage foam cell-RAW 264.7 induced by oxidized low-density lipoprotein (ox-LDL), which the rates of cholesterol efflux were 74.11% and 82.95%, respectively. In comparison, the effects were almost similar to the positive control drug-Simvastatin. Collectively, our data sμggest PEPE can be used as a valuable functional food additive for hyperlipidemia treatment.At the same time we have the two components throμgh the Fourier infrared, nuclear magnetic resonance (NMR) spectroscopy, gas chromatography, periodic acid such as oxidation experiment polysaccharides molecular weight,configuration, monosaccharide composition and proportion, as well as the monosaccharide connections between key related structure information. For the two kinds of polysaccharides mainly into beta (1-3) connecting key configuration, also contains a (1-6) connection pyran glucan, including PEPE-1 average molecular weight 37.50 kDa, PEPE-2 average molecular weight 30.00 kDa. Two kinds of polysaccharides monosaccharide composition mainly for glucose, contain a small amount of mannose and galactose.In vitro biochemical test results show PEPE-1, and PEPE-2 OH radicals has a clearance rate of 35.3% and 32.1% respectively, while PEPE-1 and PEPE-2 has a strong DPPH radical scavenging ability on DPPH radical scavenging rate reached 84.6% and 83.07%, respectively, the results show that the two single component isolated and purified from Pleurotus eryngii polysaccharide PEPE-1 and PEPE-2 has a strong antioxidant activity. Findings presented in this report firstly provide direct evidence, which links the purified polysaccharide moiety with the biological function in foam cells model. Our findings indicate that the excreted polysaccharide can disrupt the foam cell formation throμgh inhibiting the oxLDL uptake. |
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