Extraction And Separation Of Polysaccharides From Pleurotus Eryngii And Evaluation Of Their In Vivo And In Vitro Activities

Edible mushroom polysaccharides have great development and application prospects.This paper aimed to separate Pleurotus eryngii polysaccharides by low temperature water extraction method using fruiting body as raw material,and then to obtain different polysaccharide components by gradient alcohol precipitation method.The qualitative and quantitative analysis of the monosaccharide composition was carried out using high performance liquid chromatography(HPLC)for different alcoholprecipitation components.The polysaccharides were characterized by Fourier transform infrared spectroscopy(FT-IR),X-ray diffraction and thermogravimetric(TG)analysis.And the ability to scavenge hydroxyl radicals and superoxide anion was also studied in vitro and in vivo.Mouse liver and spleen index,immune factors(IgG,IL-6,IL-10,TNF-α),blood sugar content,lysozyme activity,and intestinal flora changes were also evaluated in vivo.Results: The enzymatic hydrolysis conditions for Pleurotus eryngii polysaccharide extraction were as follows: 0.04% cellulose(w/v),9.5% pectinase(w/v),54 °C,2.5 h,extraction once,with polysaccharide yield 9.93%.The hydroxyl radical scavenging ability of the polysaccharides were greatly dependent on the kinds of polysaccharides obtained by different ethanol precipitation.Mice treated with polysaccharides undergone traditional alcohol precipitation(group A),60% ethanol alcohol precipitation(group D)and 80% ethanol alcohol precipitation(group E)showed improved antioxidant capacity.Pleurotus eryngii polysaccharide increased the serum IgG content and lysozyme activity significantly in mice.The results showed that the different polysaccharides precipitated by gradient ethanol solution had different monosaccharides composition,which revealed different potential in improving the non-specific immunity of mice.These Pleurotus eryngii polysaccharides exhibited different immune potentials by regulating cytokines and intestinal flora,which were closely related to the monosaccharide composition of different polysaccharides.