Study On The Mechanism Of "poison - Effect" In Ascites Rats

Objective"Eighteen Antagonisms" is the content of the incompatibility of Chinese medicine theory, anti drug combination is absolutely contraindicated for clinical use, ancient physicians disagree, some physicians revered ancient precept think clinical should avoid the use of anti drug, and physicians and that anti drug can be used at the same time. Anti toxicity and efficacy of the combination of drugs may be related to anti drug dosage and proportion, variety, processing, medicine way and times of administration and other related. This national key basic research and development plan (973) set up "based on special (project number: 2011CB505300) "Eighteen Antagonisms " the compatibility of traditional Chinese medicine taboo on the theoretical basis of to explore the" Eighteen Antagonisms anti incompatibility theory, which task group to assume the task of six" based on the clinical application of traditional Chinese medicine taboo conditions and compatibility relations research" (project number:2011CB505306) with kansui licorice anti drug combinations of Zhang zhongjing name kansui-banxia decoction as the carrier, choose cancer ascites as a model, to investigate kansui licorice anti drug combination in accordance with the "toxicity" effects of different compatibility conditions caused by. Our team has done some Preliminary exploration of the combine with Gansui and Banxia in ratio, dose, medicine concocted methods and varieties of herb,based on the fufang of Gansui with Banxia decoction from Zhang zhongjing. In order to conclude the compatibility and incompatibility of use of combination of anti-drug.In view of the Pharmacopoeia of the people’s Republic of China records Gancao has three different kinds which is Glycyrrhiza uralensis Fisch., Glycyrrhiza inflate Bat. or Glycyrrhiza glabra L.But the compound of Gansui with Banxia decoction from Zhang zhongjing has not record the species of Gansui.Therefore,our study based on this Classic Prescription to establish the malignant ascites model of rats.Based on Previous research, investigate the influence of liver and kidney tissue on gansui with banxia decoction,or without one or two herbs in this decoction, under the condition of different varieties of Gancao.In order to investigate the toxicity and efficacy on Gansui with Banxia decoction whether has the influence of the combination of anti-drug or the species of gancao.Eventually, to make a supplement and improvement of theoretical study on"Eighteen Antagonisms".To provide scientific support for clinicians to prescrib anti-drug combinations of gansui with gancao have reasonable security and have the further improve anti-drug compatibility and incompatibility incompatibility of use this combination.MethodsOur team has found gansui and banxia in 0.11g:1.67g condition has good diuretic effects and play a protective role on the tissue of liver and kidney. It is the appropriate experimental conditions..In order to investigate the toxicity and efficacy on Gansui with Banxia decoction whether has the influence of the combination of anti-drug or the species of gancao.Experiment set up blank group, model group, positive control group, the group of Glycyrrhiza uralensis Fisch., the group of Glycyrrhiza inflate Bat., the group of Glycyrrhiza glabra L. the group of Glycyrrhiza uralensis Fisch.without gansui, the group of Glycyrrhiza inflate Bat. without gansui, the group of Glycyrrhiza glabra L. without gansui, the full party group without gancao, the full party group without gansui and gancao. A total of 11 groups,11 days after the drug was compared between the groups.This research made a longitudinal comparisons between groups under the condition of gansui combine with three different species of gancao,or without one or two herbs in the decoction. In order to investigate the toxicity and efficacy on Gansui with Banxia decoction whether has the influence of the combination of anti-drug or the species of gancao.Results(1)toxicity characterizationCytochrome P450 enzymes in liver: ① Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:the Glycyrrhiza uralensis Fisch. group, the Glycyrrhiza inflate Bat. group, the Glycyrrhiza glabra L. group will promote the expression of CYP3A1mRNA CYP3A2mRNA. The Glycyrrhiza uralensis Fisch. And the Glycyrrhiza inflate Bat. group increased with significant difference (P< 0.05 or P< 0.01). ② Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:Glycyrrhiza uralensis Fisch. Group and Glycyrrhiza inflate Bat. Group inhibition of CYP2E1mRNA expression is superior to remove one or two kinds of anti drugs in Gansui-Banxia compound, with significant difference (P< 0.05 or P< 0.01), The Glycyrrhiza uralensis Fisch. Group in promoting CYP3A1mRNA, CYP3A2mRNA expression is superior to one or two kinds of anti drugs in Gansui-Banxia compound, which the group of Glycyrrhiza uralensis Fisch. without gansui, the full party group without gansui and gancao,increased with significant difference (P< 0.05 or P< 0.01).The Glycyrrhiza glabra L. group in promoting the expression of CYP3A1mRNA weak on remove one or two kinds of anti drugs in Gansui-Banxia compound,with statistical difference (P< 0.01). Glycyrrhiza inflate Bat. Group is to promote the expression of CYP3A2mRNA outperforms all removed one or two kinds of anti drugs in Gansui-Banxia compound, with statistical difference (P 0.01).Kidney tissue Apoptosis:① Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Glycyrrhiza uralensis Fisch. group, Glycyrrhiza inflate Bat. group, Glycyrrhiza glabra L.group can inhibit renal cell apoptosis than those in the model group, with statistical difference (P< 0.01).②Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:Glycyrrhiza uralensis Fisch. group in the inhibition of renal cell apoptosis is superior to full remove one or two kinds of anti drugs in Gansui-Banxia compound, with statistical difference (P< 0.01). Glycyrrhiza glabra L.group in the inhibition of renal cell apoptosis is superior to full remove one or two kinds of anti drugs in Gansui-Banxia compound, which the full party group without gancao, the full party group without gansui and gancao, increased with significant difference (P< 0.05 or P< 0.01). Glycyrrhiza inflate Bat. group in the inhibition of renal cell apoptosis is superior to one or two kinds of anti drugs in Gansui-Banxia compound, which the full party group without gansui and gancao group increased with significant difference (P< 0.01).(2)Efficacy EvaluationKidney AQP-2 enzyme and protein:①Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Compared with the model group the Glycyrrhiza uralensis Fisch. group, Glycyrrhiza inflate Bat. group could inhibit the expression of AQP-2mRNA and protein. ②Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:Glycyrrhiza inflate Bat. group and Glycyrrhiza glabra L. group were weaker than the full party group without gancao group in inhibiting the expression of AQP-2mRNA, with significant difference (P<0.01).Glomerular filtration rate:①Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Compared with the model group the group of Glycyrrhiza uralensis Fisch., the group of Glycyrrhiza inflate Bat. and the group of Glycyrrhiza glabra L. decreased glomerular filtration rate.②Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:The Glycyrrhiza uralensis Fisch. group in the increased glomerular filtration rate is superior to the group of Glycyrrhiza uralensis Fisch.without gansui, and the full party group without gancao.Free water clearance: ①Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Compared with the model group the Glycyrrhiza uralensis Fisch. Group CH2O showed a decreasing trend.The Glycyrrhiza inflate Bat. group and Glycyrrhiza glabra L. group showed increasing trend.②Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:The Glycyrrhiza uralensis Fisch. group reduction of CH2O in group was superior to the group of Glycyrrhiza uralensis Fisch.without gansui.The Glycyrrhiza inflate Bat. group and Glycyrrhiza glabra L. group in reducing CH2O is superior to remove one or two kinds of anti drugs in Gansui-Banxia compound.Urinary epidermal growth factor: ① Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Compared with model group, the group of Glycyrrhiza uralensis Fisch., the group of Glycyrrhiza inflate Bat.and the group of Glycyrrhiza glabra L.of rat urine EGF showed rising trend.② Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:The group of Glycyrrhiza uralensis Fisch. in promoting the concentration of EGF in urine than the group of Glycyrrhiza uralensis Fisch.without gansui, the full party group without gancao.The Glycyrrhiza inflate Bat. group to promote the concentration of EGF in urine was superior to the full party group without gancao group.Urine beta-2 microglobulin: ①Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Compared with model group, the group of Glycyrrhiza uralensis Fisch., the group of Glycyrrhiza inflate Bat. and the group of Glycyrrhiza glabra L. of rat urine β 2-MG showing a decreasing trend.②Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:The Glycyrrhiza uralensis Fisch. group in reducing the rat urinary beta 2-MG is superior to the group of Glycyrrhiza uralensis Fisch.without gansui.Urinary albumin: ① Under the condition of Gansui with Banxia decoction which contain gansui and different kinds of gancao:Compared with model group, the group of Glycyrrhiza uralensis Fisch., the group of Glycyrrhiza inflate Bat. and the group of Glycyrrhiza glabra L. of rat urine Alb showed a decreasing trend.② Under the condition of Gansui with Banxia decoction which add or subtract gansui and gancao:The group of Glycyrrhiza uralensis Fisch., the group of Glycyrrhiza inflate Bat. and the group of Glycyrrhiza glabra L. in lower ALB content is superior to remove one or two kinds of anti drugs in Gansui-Banxia compound.Conclusion(1) Gansui with different varieties of Gancao anti drug combination in Gansui-Banxia decoction Taboo conditionsPreliminary think the Glycyrrhiza uralensis Fisch. group, Glycyrrhiza inflate Bat. group and Glycyrrhiza glabra L.were the suitable conditions of compatibility. Because of the compatibility of the one hand, it will promote cancerous ascites in vivo metabolism, reflecting better diuresis; on the other hand will promote the metabolism of toxic substances, slow down the tissue cell apoptosis and on the damage of liver and kidney to protective and repairing effects. Glycyrrhiza uralensis Fisch. group in promoting cancer ascites metabolism, Liver and kidney protective effects were better than the group of Glycyrrhiza inflate Bat., the group of Glycyrrhiza glabra L., belonging to the compatibility of "efficiency".(2) Gansui with different varieties of Gancao anti drug combination in Gansui-Banxia decoction Add and subtract gansui and gancao medication Taboo conditionsContain Glycyrrhiza uralensis Fisch.and gansui in Gansui-Banxia decoction remove one or two kinds of anti drugs group, Glycyrrhiza inflate Bat. and gansui in Gansui-Banxia decoction remove one or two kinds of anti drugs group,improve liver cytochrome P450 enzyme expression is weak than Glycyrrhiza uralensis Fisch. group and Glycyrrhiza inflate Bat. group.Contain Glycyrrhiza uralensis Fisch.and gansui in Gansui-Banxia decoction remove one or two kinds of anti drugs group, Glycyrrhiza inflate Bat. and gansui in Gansui-Banxia decoction remove one or two kinds of anti drugs group, Glycyrrhiza glabra L. and gansui in Gansui-Banxia decoction remove one or two kinds of anti drugs group in the diuresis function, renal AQP-2 expression regulation aspects compared with Glycyrrhiza uralensis Fisch. group, the Glycyrrhiza inflate Bat. group and Glycyrrhiza glabra L.group did not exhibit the role of a good trend.