| Purpose:Based on the theory of "spleen main muscle limbs".the present experiment is designed to determine the effects of medicated food on Sarcopenia related apoptotic signaling in rectus femoris muscles of D-galactose aging model rats after medicated food invention to better understand the cellular and molecular etiology due to apoptetic signaling pathway,and to provide safe and effective nursing measures and clinical intervention of sarcopenia, to improve the quality of life of the elderly and reduce the cost of health care finally.Material and method:30 male Sprague-Dawley (SD) rats of clean grade (3 month old) were divided into 3 groups randomly:young control group(YC), aging model group(AM), aging medicinal diet group(AD)with 10 rats in each group. The rats of young control group and aging model group were treated with daily subcutaneous injection of saline(125mg-kg"1-d"1) and 7.5 percent D-gal(125mg-kg-1·d-1) respectively for 6 weeks. After the process of model, AD groups performed medicinal diet gavage(10ml·kg-1·d-1) for 4 weeks while YC&AM groups served equal volume of rice soup. After intraperitoneal anesthesia, blood from the abdominal aorta, to quickly separate the rectus femoris muscle. Under light microscope,to observe the changes of aging rat rectus femoris muscle cell morphology; Detection of serum superoxide dismutase (SOD) and SOD, Caspase-3, Caspase-8 of rectus femoris activity by Elisa; Detection of the expression of Caspase-3 and Caspase-8 of rectus muscle by PCR.Results:1.General parameter:The rats of AD and AM group showed up the aging appearances, the body weight were in normal increase trend, and all the rats in aging medicinal diet group could slow down the above trend; Results indicated that medicinal diet interference way could improve the aging appearances effectively.2.Skeletal muscle tissue slice:Under light microscope, the rats in group YC were normal skeletal muscle histologic characteristics. The rats of M and MD group showed up muscle cell atrophy or regressive changes in a different level, especially the rats in group AM. Compared with group AM, the muscle cell atrophy or regressive changes were lower in AD group.Results indicate that medicinal diet could prevent the premature appearance in skeletal muscle aging.3. Serum parameter:Compared with group YC, the serum SOD in group AM were lower(P< 0.05); the AD group’s SOD active level was significantly higher than the AM group’s (P< 0.01).Results indicated that the serum SOD enzyme activity increased after medicinal diet interference.4.Skeletal muscle biochemical parameter:Compared with group YC, the skeletal muscle SOD in group AM were lower (P<0.01), but Caspase-3&Caspase-8 enzyme activity increased; Compared with group AM, AD group’s SOD active level was significantly higher and Caspase-3&Caspase-8 enzyme activity were lower(P<0.01). Results indicated that the serum SOD enzyme activity increased after medicated diet interference. Besides, medicinal diet could also improve the ability of scavenging oxygen free radicals in skeletal muscle and reduce apoptosis.5.Skeletal muscle molecular biological parameter:Compared with group YC, the skeletal muscle Caspase-3&Caspase-8mRNA in group AM were higher(P<0.01). Then compared with group AM, AD group’s Caspase-3&Caspase-8mRNA contents were significantly lower. Results indicated that medicinal diet could reverse the trend of Caspase-3&Caspase-8mRNA contents.Conclusion:1. Diet intervention can improve the appearance of aging rats and skeletal muscle pathological morphology, which is close to normal, so it is concluded that the diet intervention has good effect on Sarcopenia.2. Diet intervention can increase the activity of superoxide dismutase (SOD) in rat serum and skeletal muscle, and improve the antioxidant capacity of skeletal muscle and reduce oxidative damage, thus delaying skeletal muscle aging.3. Diet intervention can decrease the apoptosis of skeletal muscle cells in Caspase-3, Caspase-8 activity and expression, and thus weaken the apoptosis, slowing down skeletal muscle aging. |
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