Biological Effects Of High Voltage Static Electric Field On Spirulina Platensis

In this paper, the spirulina platensis were treated with high voltage static electric field, the biomass indexes of the treated algae strains ES-x(x=1-9) were compared with the original strain IS to investigate the biological effect of high voltage static electric fieled on spirulina platensis. The rusults indicated that the content of malondialdehyde (MDA) decreased, the growth speed increased significantly except ES-5, the content of chlorophyll a, beta-carotene, phycocyanin and the SOD specific activity were increased by 37.69%,32.41%,19.26% and 7.06 respectively.The ultraviolet absorption spectrum, SDS-PAGE electrophoresis and stability of the phycocyanin were measured to study the ES-8 and IS. The results showed that the position of the maximum absorption peak was the same, the value of maximum absorption peak of ES-8 was higher than IS. The electrophoresis pattern of ES-8 and ES was in agreement, both contained two parallel bands, which were the two subunits of the alga blue protein with the size of 17000Da and 20000Da. Compared with IS, ES-8 has a better stability in phycocyanin solution when the NaCl content is lower than 4%.Orthogonal experiment was adopted to optimize the extraction of chlorophyll a and the aqueous two-phase extraction of the algae blue protein in spirulina platensis. The optimization conditions were established. The results showed that the extraction rate of chlorophyll a reached 1.545% when the solid-liquid ratio was 1:500 (g/mL), ultrasonic power was 80% and continued for 20 minutes. The purity of the algae blue protein was 3.11 and the recovery rate reached 93.07% when the concentration of PEG2000 and tassium sodium tartrate solution was 17%(w/w) and 21%(w/w) respectively, the pH value was 6.5.