Headspace Single Drop Microextraction Coupled With Chromatography For Determination Of Low Molecular Organic Amine Substances

In the recent years, more and more people pay attention to environment pollutionproblem and detection of hazardous substance. Organic amines are one of the most challengefor environmental protection in the modern world. They are contaminants that need to bestrictly controlled in the industrial hygiene supervision. Amine component can be produced inthe process of industrial production and corruption of animals and plants. Such as chemicalplant, pharmaceutical factory, leather factory, meat processing plant, dustbin and sewer and soon can all be looked as sources of amine fetid substances. Organic amines are public nuisancewhich because of their extensive contaminated area. They can cause pollution to water andenvironment. Environmental science and technology workers keep a watchful e ye on organicamines for the past few years. Therefore, developing an economic and high-effective methodfor determination of trace organic amines is focus and hot spot for environment field andsignificant for people health, environment detection&controlling, and food inspection.Food is the paramount necessity of the people. How to eat to the reliable and trust food isa major social problem which is widely concerned by persons right now. However, there areso many kinds of unhealthy food. Antibiotic vestigial has been paid few attention, but itsconsequence is very serious. It can not only harm peoples’ health indirect or indirect way, butalso can discharge into the environment which cause ecological crisis. Sulfa drugs is a kind ofcommonly used broad-spectrum antibiotics, and is widely used in aquaculture.In the second chapter, we employ headspace single drop microextraction coupled withion chromatography method was developed for the prethickening and determination of threealiphatic amines. The enrichment factors were40to73, relative recovery was97.2%to102.9%, detection limits (signal to noise ratio of3) were0.96to8.18μg/L and relativestandard deviation, according to peak area, was0.96%under the optimal conditions.In the third chapter, a novel headspace single drop microextraction coupled with liquidchromatography method was developed for determination of two arylamines including anilineand o-chloroaniline in industrial wastewater. The detection limits (signal to noise ratio of3)was5.2to7.5μg/L, relative recovery was between90.1to108.7%under the optimalconditions. The experimental results show that this method has not only high sensitivity, good reproducibility, easy operation but also is economic, environment friendly and low matrixinterference and proved that the method is feasible.In the fourthly chapter, we use ultrasonic, centrifugal and headspace single dropmicroextraction as preliminary treatment for dealing with fish flesh. Ion chromatography hasbeen applied to the determination of residues of sulfadiazine. The factors which effect thesingle-drop microextraction process were optimized. Under the optimal conditions, themeasured parameters are as follows: the relative standard deviation, according to peak area,was1.2%, detection limits (signal to noise ratio of3) was3.8μg/L, enrichment factors ofsulfadiazine was60.5with relative recovery of93.2%to103.2%.