| Laver, the strong performer of marine algae, was distributed in yellow sea andbohai sea in our country. Its production is first in the world. The laver was rich in nu-trients and active substances, including proteins, amino acid, unsaturated fattyacid,multivitamin, active polysaccharides, dietary fiber, and so on. The studies had foundthat laver has resistance mutation, antitumor, enhancing immunity, antiaging, reducinghematic fat action, et al. The laver has a large number of negative charges and largesurface area because of the composition such as polysaccharide, protein, fat, andamino group, carboxyl group, phosphoric acid group, aldehyde group, uronic acid,hydroxy, sulfuric acid root. It can enrich the lead of water. In July2011, the35thin-ternational Codex Alimentarius Commission, the standards of laver in Asia had beenappointed to be setted. And the quality and safety problems of laver were attachedimportantly by the government, enterprises and institutions, farmers and consumers,and it became the focus of the social. Moreover, the scholars studyed on the adsorp-tion of exogenous heavy metals using the characteristics of laver. Studies found thatthe living laver and nonliving laver had good adsorption effect of metals. The mainlyadsorption mechanism were ion exchange, complexation, redox, and the micro pre-cipitated. But there were little researches on the adsorption of metals of nonliving la-ver in the living body.On one hand, this research focuses on the influences of laver on thephysiologicalfunction of rats evaluated the safety of the lead in laver via toxicological methods. Onthe other hand, on the basis of related researches, this research discussed the antago-nism and advancing excretion effect of laver on living body. On one hand, the studyaimed at guding the healthy consumption concept of laver products. Besides, this re-search aimed at investigating whether the laver has the advancing excretion effect ornot and seeking a kind of natural non-toxic antagonism lead toxic substances, as wellas providing data support for the setting of lead limit. Firstly,98Wistar rats were randomly divided the into7groups (14rats for each)respectively. Group A served as the normal control group, B as the higher dosageporphyra yezoensis group (500mg/kg.bw.d), C as the middle dosage porphyra ye-zoensis group (300mg/kg.bw.d), D as the lower dosage porphyra yezoensis group(100mg/kg.bw.d), E as the higher dosage porphyra haitanensis group (500mg/kg.bw.d), F as the middle dosage porphyra haitanensis group (300mg/kg.bw.d),G as the lower dosage porphyra haitanensis group (100mg/kg.bw.d). The experimentlasted30days, on the15thand30thdays of the experiment, the rats were sacrificed andlivers, kidneys, feces and abdominal aortic blood were collected respectively. Andrecord the body weight weekly, as well as the appearance, actions and activities, diet,physical condition and toxic symptom. The metal ion levels of lead in the blood sam-ples and the organ specimens were measured through ICP-AES. Malonyldialdehyde(MDA), Superoxide dismutase (SOD), Glutathion peroxidase (GSH-PX), Total serumprotein (TP), Cereal third transaminase (ALT), Alkaline phosphatase (ALP), Aspertateaminotransferase (AST), urea nitrogen (BUN), Creatinine (Cr) were determined bykits. Secondly, the112Wistar rats were randomly divided into8groups (14rats foreach) respectively. Group A served as the normal control group, B as thelead-poisoned control group (5mg/kg.bw.d), C as lead-poisoned+porphyra yezoensistreated (at higher dosage) group, D as lead-poisoned+porphyra yezoensis treated (atmiddle dosage) group, E as lead-poisoned+porphyra yezoensis treated (at lowerdosage) group, F as lead-poisoned+porphyra haitanensis treated (at higher dosage)group, G as lead-poisoned+porphyra haitanensis treated (at middle dosage), H aslead-poisoned+porphyra haitanensis treated (at lower dosage) group. The experi-ment lasted30days, on the15thand30thdays of the experiment, the rats were sacri-ficed. And the measured indexes were same as what listed in above experiments, inorder to understand the advancing excetion effect of edible laver, and the protectiveeffect on liver, kidney and antioxidant system.Then, the conclutions were listed in the follwing:1. Porphyra yezoensis andporphyra haitanensis can promote the growth of rats and enhance the antioxidant sys-tem. Porphyra yezoensis can restrain lipid peroxidation; all the treatments of porphyrahaitanensis and the lower dosage of porphyra yezoensis can maintain and increase theactivity of SOD and GSH-PX. Porphyra haitanensis is better than porphyra yezoensisin enhancing the antioxygenation.2. Laver can protet rat’s liver. Porphyra yezoensisand porphyra haitanensis can decrease the concent of lead in liver, and the antago- nism effect is outstanding on the rats exposed to lead short time and low dosage,what’s more, porphyra yezoensis is better than porphyra haitanensis. Porphyra ye-zoensis and porphyra haitanensis can decrease the concent or activety of ALP、AST、ALT and ALT/AST. Considering all the indexes, the treatment of higher dosage ofporphyra haitanensis is best on protecting liver.3. Porphyra yezoensis and porphyrahaitanensis can decrease the content of lead in rat’s liver and kidney, and enhance themetabolism of lead. Porphyra yezoensis is better than porphyra haitanensis on de-creasing the content of lead, and the best treatment was the lower dosage of porphyrayezoensis. The lower dosage of laver (Porphyra yezoensis and porphyra haitanensis)were efficient in decreasing the lead in kidney of the rat’s exposed to lead in the shortterm. And the middle dosage and higher dosage of laver (Porphyra yezoensis andporphyra haitanensis) were efficient in decreasing the lead in kidney of the rat’s ex-posed to lead in the longer term. The best treatments were the lower dosage ofporphyra yezoensis and the lower dosage of porphyra haitanensis. Moreover, as theincreasing of the dosage of porphyra yezoensis, advancing excretion effect is stronger. |
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