| Beer is the world’s universal beverage, Chinese brewery production hasaccountcd for a quarter of the world, ranking ifrst in the world in2013, and thedischarg of brewery wastewater was over more than ifve hundred million tons.Brewery wastewater contains very typical biodegradable organic matters, every year alarge number of brewery wastewater emission aggravated the pollution ofenvironment.Typical brewery wastewater contains a high concentration of organic pollutants,due to the extremely easy degradation of contaminated materials such as sugar, starch,protein and the dififcult degradation of contaminated materials such as cyanineglycoside, lignin with phytic acid calcium. The current biological treatment methodsused in brewery wastewater general have the shortcomings of the early corruption andthe difficult falling of COD in later period, which impacts the the effect of thetreatment of brewery wastewater. Compound microbial strains mean to mix microbialstrains with different degradation based on the material composition of wastewater,which is distributed according to a certain proportion. It realized the balanceddegradation of different pollutants to improve the removal rate of COD of brewerywastewater through adjusting the degradation speed of mixed pollutants by thesynergistic effect and hydrolysising properties of the microflora, The mainconclusions are listed as follows:1. Ten strains with high production of cellulase，protease, lipase and amylasewere separated through the domestication and enrichment culture based on differentkinds of several hydrolases. The10strains had obviously different abilities to producethe enzymes. Cellulase and lipase activity of X-7strain were the highest, reaching48.22U/mL and6.47U/mL. and the protease activity of X-8strain reached up to58.63U/mL, amylase activity of X-5strain up to19.68U/mL, while the other seven strains had moderate enzyme activity. All of ten strains had short adaptation andstrong ability to reproduce.2.The proportion of the strains and the treatment conditions of brewerywastewater were optimized through the responsed surface method based on the typesand the activity of the enzymes. Through Plackett-Burman and Box-Behnkcnexperiments, it showed when X-3, X-4and X-7strains compound of4:4:3，and thestrains were put the best quantity as0.4mL/50mL, the highest removal rate of thesimulative brewery wastewater reached to91.17%，improving30.06%compared withthe matched group. Through physiological, biochemical and16s rDNA identificationof the three strains, they were respectively confirmed Bacillus sp.，Bacillus sp. Ml7，Bacillus subtilis and Bacillus sp. JNF-1.3.The compound microorganism after being optimized was applied to treat theactual wastewater, the result of which showed that the primary and secondary factorsaffecting the removal rate of COD were temperature and inoculation dose, loadingvolume and pH, that was to say, when the temperature is35°C, pH9.0，loadingvolume50mL/250mL, inoculation dose0.4mL/50mL, the COD removal rate ofbrewery wastewater treated atfer five days reached as high as70.77%, improving11.34%compared with the matched group. Then used compound bacteria to resolvethe brewery wastewater with the best conditions for14d’ays dynamic monitoring, theresult showed that the removal rate of COD, NH3-N had a sharp decline, TP risedbefore a failed trend. The removal rate of COD, NH3-N and TP of the brewerywastewater were95.38%,93.31%and77.40%after the treatment by the mixedmicroorganism and the increasing proportion were20.77%,22.18%and13.01%compared with the contrasted group.It effectively promotes the competlely removal of COD of brewery wastewaterbased on the distribution of the compound microorganism and enriches the resourceof degrading organics and sets up the module for the degradation of vairous mixedorganic pollutants. |
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