| The reserves and production of antimony in China rank among the head of the world, especially in lengshuijiang city of Hunan Province. Its said "World antimony are".Antimony mining mineral smelting and industrialized.But the resulting wastewater pollution also more serious.Because of antimony is a widespread distribution of toxic elements to the body and the creature with chronic toxicity and carcinogenicity. It seriously influenced water ecological environment and residents'safe of drinking water. So to contain the antimony wastewater pollution effective governance is urgently needed. In present the technology for the treatment of wastewater containing antimony research are relatively few. Taking lengshuijiang city xikuangshan antimony ore dressing wastewater mining area for object,On the methods of microorganism method for processing, Breeding a high-efficiency microbial strains. The study is made on the bacterium fluid handling antimony ore dressing wastewater process and its parameters.Get a conclusion that:(1) From microorganism strains of a large area of Hunan province in wastewater sediment. Through 9K. medium for inoculating culture in the laboratory. In the conditions set to cultivate the material wastewater vaccinations containing antimony wastewater treatment. Bying single factor experiment and determined the microbe treatment of wastewater containing antimony experimental parameters. Namely microbial inoculated quantity 5%, processing system pH value to 2.0, treatment of processing time is 4 days, for temperature of 30℃.(2) In the single factor experiment parameter is conducted and optimized on the basis of orthogonal design experiments.From the orthogonal experiment intuitive analysis and calculation results can be seen:Microbial inoculation quantity of 5%, pH value for 2.5, the processing time for 3d, processing temperature for 30℃, the total antimony removal rate over 99%.Effluent is superior in hunan province "industrial wastewater pollutant discharge standards (antimony DB43/350-2007) specified in the emission concentrations of antimony attained. Through that poor and variance analysis, Microbial inoculation amount to the most significant removal influence, Various factors on antimony order of sequence is microbial inoculated quantity> processing system pH value> treatment of processing time> handling temperature.(3) Using molecular biology identification method of experiment strains, then through the separation, purification and morphological analysis. Will the microbial 16S rDNA sequence Genbank (http://www.ncbi.nlm.nih.gov/) submitted to database. All of the microorganisms in the program 16s in Genbank rDNA gene sequences and already login 16s rDNA gene sequences nucleotide allogenic comparison. Identify the microbial belong to of the genus Bacillus sp.(4) Through the SEM and EDX analysis of precipitation slag after microbe treatment.For things to precipitate slag phase analysis and found in large amounts of precipitation slag enrichment of antimony. And initially deduce the of the genus bacillus microbial mainly through the cell adsorption and flocculation micro precipitation dual role removing heavy metal antimony in waste water. |
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