Developmental And Neural Effect Of Nanosilica Particles On Zebrafish

Nanoparticles are wildly employed in medical application, especially in drug delivery. Study found that nanoparticles are mainly in the form of nanoparticles-protein corona complex in vivo. Silica nanoparticles are in a wide application in industry and agriculture and pharmacy. Also, Silica nanoparticle-protein corona complex has been employed in a wide range application, but that the materials have different level of toxic effect has been an important issue. Bovine serum albumin component five (BSA-V), which is abundant in content, are employed to package the silica nanoparticles in different size (15nm and50nm) into nanoparticles-protein corona complex with different diameter and surface charge. BSA-V also have reduce the coagulation of silica nanoparticles in system water. In this study, Zebrafish larvae are employed as in vivo platform for toxic detection by evaluating the developmental and neural toxicity induced by silica nanoparticles in different size (15nm and50nm) and in a wide range of concentration by exposing embryo to silica nanoparticles-protein corona resolved in standard system water. For developmental toxicity, we applied the hatch rate, mortality,malformation and cartilaginous defects as the toxic criterion. DLS result demonstrated that BSA-V package is an effective way to prevent the silica nanoparticles coagulation and to control the nanoparticle size and charge. The hatch rate results illustrated that the concentration-dependent effect of silica nanoparticle-protein corona complex. The mortality results were increased from54hour post fertilization (hpf) to120hpf explained by the LC50values. The malformation incidence, average toxicity score of the malformations, and the vertebral column of zebrafish further supported the50nm NPs corona complex have more toxicity. The development stages of zebrafish at54hpf is a novel and important time point for evaluating the toxicity of complex. The results showed that same protein-coated NPs corona complex had different surface characters and toxic extent. The50nm NPs corona complex are more toxic than15nm NPs corona complex during the developmental stages. While the cartilaginous toxicity further confirm the developmental toxic results. Furthermore, for neurotoxicity, we defined several neurobehavioral parameters based on the sleep-wake behavior, to detect the effect of nanoparticles in different size (15nm and50nm) and different concentration (from50to100μg/mL) on zebrafish nerobehavior. The results show a concentration-dependent increase in behavioral neurotoxicity, mortality,and malformation among larvae treated with the SiO2nanoparticles of15nm and50nm. A comparison of the15nm and50nm NPs by K-means clustering analysis demonstrates that the15nm NPs have a greater neurotoxic effect than the50nm NPs, with the50nm NPs exhibiting greater developmental toxicity on the zebrafish larvae than the15nm NPs.