Study On Purification Process Of Total Flavon-oids In Semen Hoveniae Extraction And Separation

The effective components of medicinal and edible plant is an important rawmaterial for bio pharmaceutical and health food. And as natural active,ingredient-s Flavonoids has become an important research focus in biomedicalfield. Hovenia dulcis Thunb,which is rich in flavonoids,has manypharmacological effects, such as liver hangover. High purity flavonoids extractedfrom Hovenia dulcis Thunb are favored in the international market. In order tomeet the biological medicine andthe development of functional foods marketdemand and considering the low production efficiency, low production and the lowpurity in the crude production of flavonoids extracted from Hovenia dulcisThunb,this paper studied the extraction, separation and purification processing offlavonoids.This study extract hovenia dulcis thunb flavonoids by the means of the ultrasonicassist and ethanol reflux respectively, on the basis of single factor and responsesurface method, optimizing the process conditions. Two extraction methods ofoptimization of process comparison test results show that the ultrasonic assistedextraction of flavonoid yield of3.528%was achieved at a rate of3.345%ethanolrefluxing extraction, compared with the former was reduced by0.183%. Andcompared with the ethanol reflux method, ultrasonic assisted method possesses theadvantages of time saving, high efficiency, and will not bring adverse effect to theseparation of follow-up work.Through the static adsorption experiment, this topic compare the four differenttypes of macroporous resin adsorption performance of total flavonoids of hoveniadulcis thunb, selecting the type of AB-8macroporous resin which is suitable forseparation and purification of total flavonoids hovenia dulcis thumb. By thedynamic adsorption experiment, the optimum technological parameters that thetype of AB-8macroporous resin separate and purify hovenia dulcis thunbflavonoids: the sample concentration is0.4mg/ml, the sample rate of2bv/h, pH= 4; And with8BV volume fraction of70%ethanol elution velocity of1.5BV/h,total flavonoids in hovenia dulcis thunb can be basic to parsing down from AB8resin, the recovery rate will reach91.17%.This article has also established coarse extraction and purification of totalflavonoids determination of hovenia dulcis thunb dihydrogen myrica rubra pigmentcontent in high performance liquid chromatographic method, the chromatographicconditions for: chromatographic column Hypersil C18column (4.6mm250mm,5um);Mobile phase of methanol-water-phosphate (and3-0.2);The velocity of1ml/min;Detection wavelength of290nm;Column temperature25℃;Sample size of20ul. Comparing before and after purification of HPLC, and purifying the hoveniadulcis thumb extraction, which protect liver composition dihydrogen arbutuselement enrichment, the content is17.02%, the purification effect is remarkable.