Proline And Adrenaline Electrochemical Studies Of Interaction

Protein is an essential element of all cells and organizational structure. There is no protein would be no life. The basic unit are composed of protein amino acids. As one of the many bioactive macromolecules of the body, amino acids are building cells, the basic materials for repair organizations. Protein is consist of amino acids, reseach amino acids is the precondition of study protein. So the reseach of amino acids have important significance. The most amino acidshave the same construction, however, the construction of proline is different. side chain and amino formed special ring structure, makes amino conformation in peptide bond relatively stable. Adrenaline is an important material of catecholamine neurotransmitters for human and mammals, which controls the nerve system response to a series of biological and chemical processes. In this paper, we focused on the electrochemical behaviour of adrenaline after adding proline. At the same time, we assisted in quantum chemistry methods to carry out the theoretical study of hydrogen bonding complexes formed between proline and adrenaline.We study the influence of proline with different concentration on electron transfer properties of adrenaline in hydrochloric acid use cyclic voltammetry and AC impedance. And we study the influence of proline with different concentrations on electron transfer properties and electrochemistry oxidation process of adrenaline in B.R. buffer solution and physiological media KRPB of pH=7.4. Experiments show that:proline can inhibit oxidation of adrenaline, and with proline concentration increased, the ability to inhibit oxidation of adrenaline enhance. With AC impedance get consistent conclusion.Finally, we do a theoretical study about the hydrogen bonding between adrenaline and proline. We optimized their configurations using the DFT-B3LYP method of quantum chemistry. The geometries of adrenaline and the stable complexes were respectively optimized to obtain structural parameters and binding energies. The calculation results could be used to explain some experimental phenomena better. The formation of hydrogen bonds plays a protective effect on the hydroxy-adrenergic. It makes the adjacent hydroxyl (-OH) of the benzene ring remove H difficultly, reduces the energy system, increases the stability of adrenaline, inhibits the oxidation of the adrenaline to a certain extent and explains the phenomenon of the experiment combining structural parameters and energy.