Immobilized Cells Moving Bed Leaching Of High Sulfur High Iron And Low Copper Refractory Copper In The Copper

With the high-grade ores was mined, the lean ores and fine ores number are more and more.Consequently,the biological hydrometallurgy technology was widely applied for smelting the low grade ores.The high efficiencies bacteria is one of key factors in the biological hydrometallurgy.The conbined bacteria was applied for digesting the opper sulphide mineral in the topic,and the results showed that the different reactions were discovered among the different bacterias with the different characteristics and sulfur element,ferrous ion and other metal ions.Due to the cooperation relationship is exist among the bacteria for the different characteristics,so that the conbined bacterias is helpful for improving the leaching efficiencyThe T.f and T.t bacteria were chose and applied for extracing the low-grade copper sulphide mineral with high sulfur and high iron.The main aims of the topic were to investigate the efficiencies and influence factors on immobilized combinational bacteria of the T.f and the T.t bacteria for microbial leaching.Firstly,the aimed leaching microorganisms were cultivated under the acidic environment condition in the lab,and further formed combinational bacterias.And the T.f and T.t bacteria were amplified, purified and repeated training under the respective suitable cultivated conditions,the high efficiencies the T.f and T.t bacteria were abtained of good activity and purified.Then the oxidation ability of the T.f bacteria was testes and the durable ability of T.f and T.t bacteria was further developed,the high durable ability and activities leaching combinational bacteria population were achievedThen,the aimed bacteria was inoculated for the durable ability.Finally,the naturalized bacteria was immobilized onto the biocarrier self-produced and was carried out the experiment in a bioreactor self-produced.The experimental results showed that(1)he optimum combinational ratio was 1 to 2 of the T.f and T.t bacteria accordingly to the experimental results under the different situation;(2)Compared results showed that extraction ratio of the Cu2+ by the non-immobilized combinational bacteria was 28.6% within 18d,but extraction ratio of the Cu2+ by the immobilized combinational bacteria was 45.2% within the same time;(3)Compared to the single bacteria was immobilized, extraction ratio of the Cu2+ reached 45.2% in the immobilized combinational bacteria system,which was prior to the extraction ability of the single bacteria,it suggested that low-grade ore was treated using the combinational bacteria was feasible;(4) the best pulp concentrations of 10% in the leaching system based on the the pulp concentrations experiment;(5) the optimal concentration of cells inoculated of 10% in the leaching system based on the compared results on the pulp concentration experiment;(6)the optimal pH value of 10% in the leaching system;(7)The leaching rates and the ore sizes was positive relation,the leaching rates were 11.3%,28.8% and 45.2% as the ore sizes of 300μm,150μm and 75μm in 18d,respectively.In all,the optimal conditions for the immobilized leaching system with combinational bacterias were as follows:the pulp concentrations of 10%, the concentration of cells inoculated of 10%, the ore size of 75μm and pH of 2.In all,the optimal conditions for the immobilized leaching system with combinational bacterias were as follows:the combinational rate was 1:2 of the T.f and T.t bacteria,the pulp concentrations of 10%,the concentration of cells inoculated of 10%, the ore size of 75μm and pH of 2.In additon.The thesis also studied the effect of Cu²⁺ leaching efficiencies and the silver ion,the silver ion was added to the leaching system,when the silver ion concentration of 5mg/L,the leaching rate was increased by 59.23% within 18 days under optimal conditions.Because the silver ion can transform into Ag₂S which can catalyze the dissolve of the ore,and lead to the Cu²⁺ leaching rate of copper sulfides was accelerated.