The Preparation Of PMOXA Antifouling Coating On Gold Surface And The Study Of Its Application

In this paper, we aim to modify the gold surface by using a simple method. In order to obtain antifouling properties, the thiol terminated hydrophilic polymer was grafted to the gold surface, based on the specific interaction between thiol and gold, thus being able to solve the antifouling problems of gold in biosensor and biomedical areas. The previous studies found that the water soluble poly(2-methyl-2-oxazoline)(PMOXA) have extremely low protein adsorption as protein resistant materials. Compared to PEG based materials, the PMOXA modified substrate shows similar protein repellent properties and better stability in vivo. So, in this study PMOXA was used as antifouling materials. It is well known that polymer brush have excellent antifouling properties for modified substrate, especially the brush PMOXA modified surface show excellent antifouling properties. So we attempt to synthesis a series of different structure "bottle-brush" PMOXA for gold surface modification, and discuss their antifouling properties. As the above mentioned, our works are described in following:In this study, a series of well-controlled bottle-brush polymers, poly(methylacrylic acid)-g-poly(2-methyl-2-oxazoline) with monothiol-terminated group ((PMAA-g-PMOXA)-SH) were synthesized by using reversible addition-fragmentation chain transfer (RAFT) polymerization and cationic ring-opening polymerization (CROP).(PMAA-g-PMOXA)-SH were grafted to the surface of gold sensor in in situ aminolysis reaction. Cyclic voltammetric (CV), X-ray photoelectron spectroscopy (XPS), variable angle spectroscopic ellipsometry (VASE), water contact angle (CA) and atomic force microscopy (AFM) were used to characterize the copolymer modified gold surfaces. The protein resistant property was investigated by surface plasmon resonance (SPR), platelet adhesion and cell adhesion were observed by scanning electron microscopy (SEM). Compared with the bare gold surface, the (PMAA20-g-PMOXA12)-SH modified gold sensor can reduce the levels of fibrinogen (Fg), bovine serum albumin (BSA) and lysozyme (Lyz) adsorption by96.5±3.1%,85.8±5.7%, and49.4±1.6%, respectively. Meanwhile, the (PMAA20-g-PMOXA12)-SH modified gold sensor also possess excellent resistance to platelet adhesion and cell adhesion. All these data demonstrated that this simple method is feasible, and that a bottle-brush (PMAA-g-PMOXA)-SH modified gold sensor has potential application in biosensor and biomedical areas.