| Ursolic acid belongs to triterpenic acid, it has tranquihzation, anti-inflammation, strength immunity , anti-bacteria, beautiful white and anti-tumor pharmacological actions. Thus much more attention has been paid to the studies of Ursolic acid since it was experimental proved having the hope to become a new potent natural anti-cancer medicine of low-poison & high-validity by the tumor hospital of Chinese acadmy of medical in recent decades.This paper is studying on the process of isolating & purifying Ursolic acid from P.tomentosa(Thunb.)Steud. It detailedly summarize the current research situation of the original plants > development in researches of Ursolic acid in pharmacological actions and the methods of separation and purification. A practical process ilow and the optimal process of extracting Ursolic acid from P.tomentosa(Thunb.)Steud. leaves -, stems & flowers were afforded by doing lots of experment. Analysing the change regulation of Ursolic acid content in samples gathered in different periods and parts, which offer the basis for collection. The content of Ursolic acid in the product obtained was determined by HPLC, the: result is 96.9%. The process obtained is:(1) Dried powder of P.tomentosa (Thunb.)Steud. leaves were extracted with 12 times absolute EtOH for 10 hours and the EtOH solution was concentrated under reduced pressure to give a crude residue which was suspended in 15-20 times PE (Bp 60-90 ) for 3-4 times (20 hours for each times) and then purified by EtOH for 3-4 times.(2)The residue obtained was subjected to column chromatography on silica gel and eluted with MeOH/H2O gradient system (8:1) followed by recrystallization in absolute EtOH finally afforded a colorless needles.(3) The content of Ursolic acid in P.tomentosa(Thunb.)Steud. gathered in different periods was determined by HPLC the result shows that the content appears to be highest in samples gathered in September lower in July and lowest in May. |
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