Tiger Huang Jian Skin Ling Spray Preparation Process And Quality Standards

This topic uses the proven prescription that an old Chinese medicine expert treats burn and scald. According to the nature of the herbs and the principle of treating clinically burn and scald, with appropriate extracting menstruum and method develop the new medical form - spray, conduct a systematical research on preparation process and quality standard.1 .Study on preparation processTake the content of anthraquinone and emodin as the target, through the comparative experiment determin the rule that Rhizoma Polygoni Cuspidati decocts with other herbs. The result indicates that the exteractive rate of effective components is higher when Rhizoma Polygoni Cuspidati is decoted with Cortex pseudolaricis than with Flos loniceraae or Radix scutellariae. Rhizoma Polygoni Cuspidati can decocted with Cortex pseudolaricis, not with Flos loniceraae or Radix scutellariae. Take the extractive rate of baical in as the target, utilizes the double wave length measuring method to determine the content of baical in to determine the method of extracting Radix scutellariae and Flos loniceraae, The result indicate that Radix scutellariae need percolate with Flos loniceraae. Useing three orthogonal experiments to optimize that the best craft condition for Rhizoma Polygoni Cuspidati reflux with Cortex pseudolaricis is to use 6 times of quantities ethyl alcohol (75%) to reflux three times, each time is 0.5 hour and for Radix scutellariae and Flos loniceraae is to use 14 times of quantities ethyl alcohol (75%) to percolate together at the speed of 5ml every minute; use 15 times of quantities ethyl alcohol (75%) to soak flos carthami for 12 hours at the room temperature, adopt the comparative experiment to determine the craft of purification and taking shape. Use 4% gelatin solution to precipitate tanning substance and adjusts PH to prevent baical in from crystallizing and separating out,2. Study on quality standardMake qualitative specification of Rhizoma Polygoni Cuspidati and Radix scutellariae with the thin layer chromatographic analysis law, determinate the relative density, the load difference, single time sprayed quantity, etc. Carry on determination of the content of emodin and baical in in the drugs with HPLC. The relative density of the end products solution is 0.84-0.90,the load inspection for 20眑ml conforms to the tipulate of Pharmacopoeia, the average of every spray is 0.125ml. The content of emodin is not less than 0. lmg/ml and bai cal i n is not less than 0. 5mg/mlThe medical form is advanced, the route and condition of craft is simple and easy to operate, preparation method is practical, feasible, and suit for the industrial product, the quality standard is perfect, the qualitative and the quantitative method is accurate, simple, reliable, It can guarantee the quality of the end product well.