Chiral Fluorescence Derivatization Rp-hplc Analysis Of Thyroxine Enantiomers

Many chiral drugs may differ in their pharmacological activities due to the difference of stereochemistry. Therefore, rapid and sensitive methods for the resolution and quantification of the individual oprically pure enantiomers are of great importance; Chromatographic methods on both the analytical and preparative scale have become of considerable interest in recent years. Particularly in the past 20 years optical resolution by HPLC has progressed rapidly and has become a practical and useful method not only for determining optical purity but also for obtaining optical isomers. In this paper, precolumn derivateization HPLC were used to separate and analyse thyroxine.First, a review on chiral resolution by HPLC is presented. Basically, three different approaches for the liquid chromatography resolution of enantiomers and the types of chiral selector are introduced. This review also deals with the development and present state of chiral selectors and chiral recognition mechanisms for enantiomers are described. Second, thyroxine enantiomeric separation with fluorescent chiral derivatization by high performance liquid chromatography is presented. Enantiomers of thyroxine were completely resoloved with R(-)/S(+)-DBD-PyNCS(R-(-)/S-(+)-4-(N,N-dimethylaminoulfonyl)-7-(3-isothio-Cyamoaopyrrolidinn)-2,1,3-benzoxadiazole)as the fluorescent chiral deriveatization reagent on C₁₈ column. In additional the influence of the mobile phase composition, pH, the concentration of chiral fluorescent reagent on the separation factor are studied. The method with DBD-PyNCS as the chiral fluorescent reagent for the separation of thyroxine has been established. Levothyroxine Sodium Tables and thyroid tablets was analysis with fluorescent chiral derivatization by HPLC, the results is satisfactory.