Hplc Separation Of Several Chiral Drugs

Chiral separation of enantiomers by high performance liquid chromatography (HPLC) has been studied. The separation methods for enantiomers of several chiral medication with PM-β- cyclodexlrin and ovomucoid as chiral stationary phase and the mechanism of separation were discussed.Direct separation of ketoprofen enantiomers and ketorolac tromethamine salt enantiomers were separated on PM-β- cyclodextrin chiral column by HPLC. The influence factors on chiral separation were considered, including the kinds and concentration of organic modifiers, the concentration of buffer solution, the flow rate and the pH of mobile phase.The optimum mobile phase to separate ketoprofen enantiomers was methanol-0.1% triethylanine acetate (TEAA) buffer solution (40:60).The mobile phase to separate ketorolac tromethamine salt enantiomers was methanol-potassium dihydrogen phosphate (KH2PO₄)(0.01mol/L) buffer solution (35:65). The resolutions of ketoprofen enantiomers and ketorolac tromethamine enantiomers were 1.101 and 1.734 respectively. Two kinds of enantiomers were separated with PM-β- cyclodextrin as chiral stationary phase.Direct separations of ketoprofen enantiomers, cetirizine enantiomers, promethazine enantiomers and allantoin enanliomers were separated on ovomucoid chiral column by HPLC. The optimum mobile phase to separate ketoprofen enantiomers was methanol-0.1% triethylanine acetate (TEAA) buffer solution (5:95), the resolution of ketoprofen enantiomers was 1.336. The mobile phase to separate cetirizine enantiomers was acetonitrile-potassium dihydrogen phosphate (KH₂PO₄) (0.02 mol/L) buffer solution (7:93), the resolution of cetirizine enantiomers was 1.441. The mobile phase to separate promethazine enantiomers was methanol- potassium dihydrogen phosphate (KH₂PO₄) (0.01 mol/L) buffer solution (6:94), the resolution of promethazine enantiomers was 1.417. The mobile phase to separate allantoin enantiomers was methanol- potassium dihydrogen phosphate (KH₂PO₄) (0.01 mol/L) buffer solution (15:85), the resolution of allantoin enantiomers was 0.625. Four kinds of enantiomers were separated with ovomucoid as chiral stationary phase.