| Objective:To study the RUNX3 protein and TGF-β1 protein in normal ovarian tissue, ovarian cystadenoma, borderline ovarian cystadenoma and ovarian adenocarcinoma. To explore their relationship to clinicopathological factors and their clinical significance in the course of ovarian adenocarcinoma.Materials:60 surgical cases of epithelial ovarian tumor specimens from Hunan Tumor Hospital and the Maternal and Child Health Hospital of Changsha from 2005 to 2009 were collected, and no patients recieved chemotherapy, radiotherapy and immunotherapy before. Age were between 26-75 years. The pathological diagnosis after survey were as below:11 cases with ovarian cystadenoma (serous, mucinous),10 cases with borderline ovarian cystadenoma,39 cases with ovarian adenocarcinoma. And at the same time selected 15 cases with normal ovarian tissue that is resected because of benign uterine. Ovarian adenocarcinoma surgical-pathological staging was according to FIGO standards, in which 21 caseswith stageⅠ-Ⅱ,18 cases withⅢ-Ⅳ. Histologic classification was according to WHO, in which 13 cases of serous adenocarcinoma, mucinous adenocarcinoma in 12 cases,14 cases of endometrial adenocarcinoma. There were 20 cases with Ascites> 500 ml, and pelvic lymph node metastasis in 13 cases.Method:SP immunohistochemical staining was used, and RUNX3, TGF-β1 all followed the standard that brown-yellow fine particles appeared in cell cytoplasm or nuclear membrane is positive. Each slice was observed by two experienced pathologists in the field of vision under the microscope for 5 visions and counted the percentage of positive under cell high power field. Positive cells per high power field in the rate of 10% to 25% was weakly positive (+),26%～50% to 50% was middle positive (++),> 50% was strong positive (+++). All pathology imaging were analyzed by Motic Image Advanced 3.2 image analysis software and measured the optical density of positive particles automatically.5 visions were selected randomly each slice to calculate the average optical density value.Results:,(1) The positive expression of Runx3 protein in ovarian cancer was 21.8%(7/32 cases), the positive rate was significantly lower than 70% of borderline ovarian cystadenoma (7/10 cases), ovarian cystadenoma 81.9%(9/11 cases), normal ovarian tissue 80%(12/15 patients). The difference among ovarian cancer and borderline ovarian cystadenoma, ovarian cystadenoma and normal ovarian tissues were significantly different (p<0.01).The difference among borderline ovarian cystadenoma, ovarian cystadenoma and normal ovarian tissue was not significant (P> O.05). Optical density value in normal ovarian tissue (830.26±78.34) and cystadenoma (819.70±95.08) was higher than that of borderline cystadenoma (582.22±44.22) and ovarian cancer (well-differentiated:326.75±41.30, middle differentiated:69.14±17.83, poor differentiated:19.08±10.18). The expression of the optical density was related to pathological grade (p<0.05), and the relation to surgical-pathological stage, histological type, lymph node metastasis, factors such as age and amount of ascites in patients with ovarian cancer was not significant (P> 0.05). (2) The positive expression of TGF-(31 protein in ovarian cancer was 17.9%(7/39 cases) and was significantly lower than 80% of borderline ovarian cystadenoma (8/10 cases),72.7% of ovarian cystadenoma (8/11 cases), normal ovarian tissue 73%(11/15 patients). The difference between Ovarian cancer and borderline ovarian cystadenoma, ovarian cystadenoma and normal ovarian tissues was significantly different (p<0.01). The difference between borderline ovarian cystadenoma, ovarian cystadenoma and normal ovarian tissue was not significant difference (P> O.05). (3)The optical density value of TGF-β1 protein in normal ovarian tissue (459.28±45.78), and cystadenoma (478.16±52.60) were higher than that of the junction of the capsule gland (387.25±31.37) and ovarian cancer in the light density (well-differentiated:204.22±18.18, middle-differentiated:49.98±5.5, poor-differentiated:11.68±1.2). The expression of the optical density was significantly related to pathological grade (p<0.05), and the relation to surgical-pathological stage, histological type, lymph node metastasis, factors such as age and amount of ascites in patients with ovarian cancer was not significant (P> 0.05). (4) The expression of RUNX3 and TGF-β1 protein was significantly correlated (r= 0.786, p= 0.007). The expression of RUNX3 and TGF-β1 protein changes with ovarian tumor grade and showed the same trend, that is, the lower differentiation, the lower the expression.Conclusion:1, The expression of Runx3 protein and TGF-β1 protein was related to the pathological grades of ovarian cancer, suggesting that they all correlated to the occurrence of ovarian cancer.2, In ovarian adenocarcinoma, Runx3 protein and TGF-β1 protein related each other, and they may play a synergistic role in the occurrence of ovarian adenocarcinoma. |
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