Experimental Studies Of The Effect Of Il17 On The Expression Of Receptor Activitor Of Nf-kb Ligand Mrna And Osteoprotegerin Mrna In Patient Of Rheumatoid Arthritis Lymphatic Cell

To study the expression of receptor activator of nuclear factor-kB ligand(RANKL) and osteoprotegerin(OPG) on the peripheral blood lymphatic cell of the patient with rheumatoid arthritis;To explore whether IL17 increase the number and activity of osteoclast by upregulating the expression of RANKL factor and causing bone destruction of rheumatoid arthritis patients. METHODS: Isolating and culturing lymphatic cell from peripheral blood of patient with rheumatoid arthritis ,and to identify lymphatic cell by immunohistochemical methods. Isolated lymphatic cell is treated by IL17 of different concentration (10-9ug/ml ,10-8ug/ml,10-7ug/ml,10-6ug/ml,10-5ug/ml). After incubation for 24h , semi-quantitative RT-PCR was performd to detect the expression of OPG,RANKL mRNA and the ratio of RANKL/OPG usingβ-actin mRNA level as the internal control.And the 10-6ug / ml concentration group setting time (0,4,16,24,48 hours) . At different times, the total RNA was extracted using the same method to detect the expression of RANKL, OPG mRNA and the ratio of RANKL/OPG. RESULTS:After 24 hours 10-9-10-6mol/L IL17 up– regulated the expression of RANKL mRNA with dose-dependent ,but inhibited the expression of OPG mRNA. Ratio of RANKL /OPG mRNA in human lymphatic cell showed increasing tendency(P<0.05). As the extention of time,IL17 upregulated RANKL mRNA and inhibited the expression of OPG mRNA is increasingly heighten, but RANKL/OPG ratio was significantly increased. Compared with the control group, in the periods of different times changes of RANKLOPG mRNA and RANKL/OPG ratio was significantly different.(p<0.01)CONCLUSION: IL17 may regulate the differentiation and activity of osteoclasts by influencing RANKL, OPG mRNA and the ratio of RANKL/OPG mRNA,and thus indirectly regulate osteoclast differentiation and activity, causing bone destruction.