Study On Prunella Vulgaris L.extraction Interventing Impair Glucose Tolerance

Impaired glucose intolerance (IGT) is an intermediate state in the transition from normal glucose tolerance (NGT) to type2 diabetes. Now diabetes has been one of the leading causes of death. Thus the screening of new compounds including plant extracts for antidiabetic effects is mandatory.In the research of medicament, there are many evident side-effects of the synthetic medication during treatment. So screening effective and lowering side-effect medication becomes very important in Chinese herb investigation. Prunella vulgaris L. has been reported to have a wide range of health benefits in oriental medicine. The extraction from Prunella vulgaris L. Was used, function and its mechanism was researched.Orthogonal design was used to screen procedures of a-amylase inhibitor from Prunella vulgaris L. Aqueous extract and aqueous-ethanol extract was used to obtain a-amylase inhibitor from Prunella vulgaris L.Using aqueous extract,ethanol extract and Polysaccharide to test activity of a-amylase through Van Loon's method, using aqueous extract to study its inhibitory kinetics characteristic. The significant effect of aqueous extract on a-amylase was observed and relevant to amount. The activity of a-amylase inhibitor was stable in high temperature and high pH. The effects of aqueous extract and aqueous-ethanol extract were observed, and the utility part of Prunella vulgaris L. was aqueous-ethanol extract.Aqueous extract from Prunella vulgaris L. inhibit maltose decompose across mice everted gut sacs in vitro. a-glucosidase inhibitor was found in the aqueous extract, and it works in a dose-dependent manner. Evaluate of Maximum Tolerant Dose of extracts from the Prunella vulgaris L. was taken, the extract at dose of 108g/kg/d has no acute harm to mice health.This study is to examine the antihyperglycemic effects of Prunella vulgaris L. in normal ICR mice and alloxan-induced hyperglycemic mice.The effects of Prunella vulgaris L. aqueous extract on blood glucose, liver glycogen were investigated. In three doses (5.5,11,22g/kg)of extracts from Prunella vulgaris L., A significant decrease in blood glucose levels was observed after treatment of aqueous extract after 30 min in the acute starch tolerance test in normal ICR mice. The extract at dose of 22g/kg significantly suppressed the rise in blood glucose in alloxan-induced hyperglycemic mice.The significant effect, which aqueous extract delaying glucose uptaken on normal ICR mice and alloxan-induced hyperglycemic mice, was oberserved. The mechanism of Prunella vulgaris L. aqueous extract may relevant toα-glucosidase inhibitor. Though Prunella vulgaris L.is not an usual antidiabetic Chinese herb, mechanism in delaying glucose uptaken was worthy to study.