Detecting Dna Damage In Workers Occupational Exposure To Lead With γh2ax Flow Cytometer Assay

Objective:To study whether lead exposure can introduce DNA damage with flow cytometer(FCM) assay.To explore the different route of exposure,dose-response relationship by different types of work on the comparison of DNA damage.To explore genetic toxicity of occupational lead exposure.To study whetherγH2AX flow cytometer assay is a sensitive,objective and effective method of peripheral blood lymphocytes DNA damage in workers occupational lead exposure.Method:The lymphocytes were obtained from 41 cases of workers occupational exposure to lead and 50 cases of control group.Flow cytometer(FCM ) assay was used to detect DNA damage.Results:1.DNA damage rate and geometric mean fluorescence intensity in occupational group was significantly higher than that in control group(p<0.05). 2.There was no statistical significance of the difference on DNA damage rate and geometric mean fluorescence intensity between different age groups(p>0.05).3.There was no statistical significance of the correlation analysis between DNA damage rate and blood lead,urine lead,δ-ALA(p>0.05).There was also no statistical significance of the correlation analysis between geometric mean fluorescence intensity and blood lead,urine lead,δ-ALA(p>0.05).4.Blood lead,δ-ALA of high concentration group and DNA damage rate were positive correlation,blood lead of high concentration group and geometric mean fluorescence intensity was positive correlation,and the correlation coefficient r had statistical significance(p<0.05).Conclusions:Occupational lead exposure can cause DNA damage.γH2AX flow cytometer assay is a sensitive,objective and effective method of peripheral blood lymphocytes DNA damage.