The Function Of Mitosis Plain Protein Tyrosine Kinase's Activation Of Protein Tyrosine Kinase Under The Influence Of Zhu-huang Kelijiâ…¡

Objective:there are two main purposes of this paper:one is to explore the activity of protein tyrosine kinases which consist in psoriasis'keratin-ocyte; the other is to explore the function of Mitosis plain protein tyrosine kinase's activation of protein tyrosine kinase under the influence of Zhu-Huang KeLiJiâ…¡.Method:Methods:the main method adopted in this paper is:first, to distribute 40 clinical cases of psoriasis sufferers completely random into two groups of subjects, a group of psoriasis sufferers and a group of ZhuHuangKeLiJiâ…¡, each group with 10 memebers, meanwhile,20 healthy volunteers are included; then to gather their skin tissues to foster keratinocytes, whose types are identified through the usage of immunity tissue; third, employing Stream style cell instrument to check the expression of protein tyrosine kinases for the purpose of comparing the differences of the state of phosohoryle of keratinocytes's keratinocyte between the psoriasis sufferers and the healthy persons; fourth, adopting western blitting to observe the influence of the extract of ZhuHuang KeLiJiâ…¡on the condition of protein tyrosine kinase s activation of Mitosis plain by exerting the extract on psoriasis sufferers'keratinocytes and adding different gradients (1,10. 100IU/ml) of concentration into the extract for 5 minutes respectively and different time gradients (1,2,5,15 minutes).Outcomes:(1)the fostered the three groups F=77.99,P<0.01,are of prominent statistical significance;(2) compared with the normal groups, the expressing ratio of the group of psoriasis sufferers raises (p<0.01); compared with the group of psoriasis sufferers, the expressing ratio of the group whose keratinocytes have been dealt with the extract succus of ZhuHuangKeLiJiâ…¡decreases (P<0.01); but there is prominent statistic of expressing ratio between the group of ZhuHuangKeLiJiâ…¡and the normal one (P<0.01);(3)the protein phosohoryle level of keratinocyte presents a gradually falling trend if acted with 50ul ZhuHuangKeLiJi II for 1 minute,2 minutes,5 minutes and 15 minutes respectively, and that level will also present a gradually falling trend if acted for the same 5 minutes but adopting 20ul,50ul,100ulZhuHuangKeLiJiâ…¡respectively, which show concentration and time dependence.Conculsion:(1)Since the protein tyrosine kinase's activity of psoriasis keratinocyte is averagely higher than that of normal group, the abnormity of the activity of protein tyrosine kinase will definitely cause the abnormity of multiplication and differentiation of the keratinocytes, thus affecting the occurring of keratinocyte. (2) The ZhuHuangKeLiJiâ…¡'s influence upon psoriasis keratinocyte will restrain the activation of Mitosis plain keratinocyte and the hyperplasia of keratinocyte, further more, there exhibit a positive relativity between its strength of anti-hyperplasia and the gradient of concentration.