Expression Of Cd4⁺cd25⁺cd127^low Regulatory T Cells And Tgf-β1 In Patients With Idiopathic Thrombocytopenic Purpura

Objective: The present study was designed to evaluate the changes and the clinical importance of CD4⁺CD25⁺CD127^low regulatory T cells,the level of TGF-β1 in peripheral blood of the patients with idiopathic thrombocytopenic purpura,and to analyze whether they have relations。To investigate they in pathogenesis of ITP and to provide a new way for ITP immunotherapy。Methods: The population of CD4⁺CD25⁺CD127^low regulatory T cells in the peripheral blood of 31 ITP patients and of 25 healthy donors was evaluated by flow cytometry,and analyse the difference of CD4⁺CD25⁺CD127^low regulatory T cells between the two groups。Secondly,drowing off 3 mL peripheral blood by tubes,quiescing for 30-60 minutes,and centrifuging at 2900 r/min for 7minutes to collect the serum, ELISA was used to test the level of TGF-β1 in blood serum。Finally,analysed the correlation between levels of CD4⁺CD25⁺CD127^low regulatory T cells and TGF-β1。Results: ITP patients had a lower proportion of CD4⁺CD25⁺CD127^low regulatory T cells than healthy controls(P<0.05),the level of TGF-β1 in the patients with idiopathic thrombocytopenic purpura was also lower than healthy controls(P<0.05),There was not positive correlation between levels of CD4⁺CD25⁺CD127^low regulatory T cells and TGF-β1 ( p>0.05 )。Conclusion: The results indicate that the decreasing of CD4⁺CD25⁺CD127^lowregulatory T cells in ITP patients may be connected with cellular immunity disturbance of idiopathic thrombocytopenic purpura.。Further Research need to be perforrmed in metabolic changes on CD4⁺CD25⁺CD127^low regulatory T cells and TGF-β1 in patients with idiopathic thrombocytopenic purpura.。...