Changes In Rats With Obstructive Jaundice Of Nf-��b And The Liver Immune Response

AIM: To investigate the variation of NF-κB and its effect on immune response in the rats with obstructive jaundice (OJ).METHODS: Sixty male Wistar rats were randomized into 3 groups of the sham operated, common bile duct ligated (CBDL), CBDL+PDCT. Liver histopathology, level of serum TB, ALT and LPS, liver IL-1β, IL-6, IL-10 and NF-κB were detected at 7d and 14d after operation respectively in each group (n=10).RESULTS: In CBDL group all the rats were injured with the liver histological damage, in which the level of serum TB,ALT,LPS as well as the expression of liver IL-1β, IL-6, IL-10 and NF-κB were all increased as compared with those in SHAM group in time of postoperation 7d and 14d (P<0. 01) and the one at 14d were more significantly (P<0. 01). After administered PDTC, as compared with those in CBDL group, the level of serum TB,ALT as well as the expression of liver IL-1β, IL-6 and NF-κB were all significantly decreased at 7d (P<0. 01), while at 14d there was no significant difference. However, after administered PDTC, as compared with those in CBDL group, there was no significant difference for the level of LPS and IL-10 at both 7d and 14d.CONCLUSION: In the early days (7d), PDTC does play a protective role in OJ by inhibiting the activation of NF-κB and down-regulating IL-1βand IL-6, so as to alleviate the injuries of liver tissues. While in the late days (14d), PDTC plays no significant effect, which may be caused by LPS, IL-10 and other functional channels.