Acrylonitrile On Vascular Endothelial Function And Its Prevention Research

Acrylonitrile (ACN), a chemical with important commercial uses, is multisystemic toxicum. Except for being involved in tumorigenesis, ACN would induce the impairment in respiratory system, digestive system, nervous system and reproductive system.As one of the most important substances which relax the vascular in vivo, Nitric Oxide (NO) plays an important role in regulating vascular endothelium function. Nitric oxide synthase catalysis L-Arg to form endogenous NO. There are three isomerides among them inducible nitric oxide synthase and endothelial nitric oxide synthase involved in vascular endothelium functions. iNOS and eNOS were detected for get message of vascular endothelium function.We explored the effect of acrylonitrile on vascular endothelium function by detecting NOS in blood serum and aorta in rat. Secondly, we explored the influence of milk and coenzyme Q10pretreatment to acrylonitrile on vascular endothelium functions in rat.Part One:The effect of acrylonitrile on vascular endothelium function. The experiment was completed by the method of gavage exposure in rats. Three groups of Sprague-Dawley rats were exposed to liquid via intragastric administration with water, maize oil or ACN (25mg/kg·bw) randomly on working day for 3 months To reflect vascular endothelium function, nitric oxide synthetase activities were detected after sacrifice.There are no significant on blood fat, hs-CRP proendotheliocyte or endotheliocyte between control group and A group (P>0.05). However the activity of iNOS of blood serum in A group (42.90 U/ml) are higher than control group (21.85U/ml) (P<0.05), there are significant between them. The activity of iNOS of aorta in A group (0.812 U/mgprot) are higher than control group (0.540 U/mgprot) (P<0.05), and there is no significant on the activity of eNOS of aorta between control group (0.371 U/mgprot) and A group (0.380 U/mgprot).Part Two:Objective to research on the influence of milk and coenzyme Q10 pretreatment to acrylonitrile on vascular endothelium function in rats.The 80 rats (in half respectively male and female) were randomly divided into 4 groups:Control group (Con), acrylonitrile exposed groups (ACN), with milk and acrylonitrile exposed groups (M+ACN), with coenzyme Q10 and acrylonitrile exposed groups (Q10+ACN). M+ACN group and Q10+ACN group were pretreated by milk and coenzyme Q10 in 30 minutes before acrylonitrile exposure, and then the acrylonitrile was given to four groups:0,25,25 and 25mg/kg-bw. After 12 weeks of exposure, the vitality of in serum and aorta total nitric oxide synthase (NOS), inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS) were measured.There are significant between Con group, ACN, M+ACN, Q10+ACN group on the activity of iNOS and eNOS (P<0.05). Comparing to Con group (21.85U/ml), the activity of blood serum iNOS are higher in ACN,M+ACNand Q10+CAN group (42.90,26.51,6.69U/ml) P<0.05). Comparing to group ACN, the activity of blood serum iNOS are lower in M+ACN and Q10+ACN group (P<0.05). Comparing to Con group(0.540 U/mgprot), the activity of aorta iNOS are higher in ACN,M+CAN and Q10+CAN group(0.812,0.773,0.622U/mgprot) (P<0.05). Comparing to group ACN, the activity of aorta, the activity of iNOS are lower in M+ACN and Q10+ACN group (P<0.05). Moreover, the activity of aorta eNOS in Q10+CAN group (0.471 U/mgprot) is higher than Con,ACN or M+ACN group (0.371,0.380,0.425 U/mgprot).Our research shows that chronic administration of ACN by gavage resulted in impairments in cardiovascular system by producing iNOS. Milk and coenzyme Q10 can slow the acrylonitrile injury on vascular endothelial function.