| Rheumatoid arthritis is a chronic autoimmunity disease mainlycharacterized as periphe))))ral symmetric and polyarticular inflammation, it is difficult to crue totally, having progressive disability and increased mortality. Neither the cause nor the mechanism of RA is entirely ciear. It has been known that immune system has close relations with the disease. Some parameters of the immune system can suggest the changes and developments of RA.Sanhuoxiaobigao (SHXBG) is medicine experience of GuangZhou university of Chinese medicine hospital YangShunYi professor external treatment rheumatoid arthritis. The medicine has blood stasis removing, eliminating dampness effects, to the clinical curative effect of rheumatoid arthritis and accurate. GuangZhou university of traditional Chinese medicine new drug development center and Vietnamese troops traditional hospital cooperation, develop new drug research SHXBG, application of modern extraction technology and modern emulsification technology will transform into preparation O/W ointment, changed the traditional Chinese medicine coarse, big, black appearance. This thesis applied the model of inflammation and pain in animal experiments to study the anti-inflammatory analgesic SHXBG for symptoms of acute and chronic inflammation. Comprehensive evaluation effect analgesic anti-inflammatory and removing blood stasis of SHXBG, provide pharmacodynamic basis for clinical treatment of rheumatoid arthritis.Purpose:Investigate the effects and mechanisms of SHXBG on rheumatoid arthritis.Method: 1 Anti-inflammatory effectMake Wistar maleness rats Adjuvant-Induced Arthritis which were derived by Freund's complete adjuvant as investigation object. Observes around the immunity tip of toe swelling, the body weight, in blood IL-1βand TNF-αinflammatory cell factor, in blood's MDA content and SOD vigor.Make the carrageenin causes the foot swelling of the big mouse as investigation object, judgment to non-specificity inflammation influence. The influence of mouse ear swelling.The influence of big mouse lint ball granuloma and the influence of mouse abdominal cavity blood capillary permeability.2 Analgesic effectSelect mouse as investigation object, with hot plate method and stretching method, make the estimate index of analgesia effect through pain threshold value of mouse, and observe the analgesia effect of Sanhuoxiaobigao.3 Eliminate stasis-relieve swelling effectThe influence of big mouse traumatic ecchymosis experiment and the influence of mouse blood stasis model experiment, observe the eliminate stasis-relieve swelling effect of Sanhuoxiaobigao.Result:SHXBG has conspicuous anti inflammatory,analgesia,immunological effect, and can patency restrain polyarticular arthritis and engorgement of AA rat's voix pedis, raise pain threshold value of AA rat. SHXBG produce a marked anti inflammatory and immunological effect through restraining secretion of too high phlegmonosis cytokines TNF-a, IL-1 of AA rats. Strengthens the SOD activeness, to reduce the MDA content. Suppresses foot which the carrageenin causes swelling. Suppresses the mouse auricle swelling. Reduces inflammation response which the lint ball produces. Improves blood capillary's permeability,reduces inflammatory exudation which the abdominal cavity the acetic acid causes. increase the analgesia effect with hot plate methodand stretching method, delay the latency response. Reduce high blood and shortening the time of disappear. The above effect of SHXBG are in proportion to its dosage.Conclusion:SHXBG has improve effect of Adjuvant Arthritis model, can improve blood stasis state of blood stasis rats, have good analgesia, anti-inflammatory and eliminate stasis-relieve swelling effect. To further study the SHXBG is provided certain rationale to treatment rheumatoid arthritis. |
PDF Full Text Request