Tangke Decoction Research On The Impact Of Type 2 Diabetic Rat Islet ¦Â-cell Apoptosis And Bcl-2 And Bax Expression

Purpose: To study the influence of TangKe decoction of compound Chinese herbs on the apoptosis of isletβcells and apoptosis-related Bcl-2 and Bax expression in type 2 diabetes mellitus rats.Material and method: Rat model of type 2 diabetes mellitus was duplicated by using small dose strepozotocin(STZ) and high caloric diet,and using TangKe decoction of compound Chinese herbs and rosiglitazone acted on model rats individually. By using methods of homeostasis model assessment of isletβcells (HOMA-β),hematoxylin-erosin pigment,Terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL),Stretavidin proxidase method (SP) and optical microscopic observation,to measure the function of HOMA-β,observe the morphology of isletβcells,the positive expression of apoptosis of islet cells and apoptosis of isletβcells apoptosis-related Bcl-2 and Bax expression in type 2 daibetes mellitus rats.Results:1. HOMA-β: Before the treatment,model group was significantly lower than normal control group level in HOMA-β(P<0.01).After the treatment,the levels of HOMA -βof TangKe decoction of compound Chinese herbs group and rosiglitazone group are significantly lower than the normal group,but they are obviously higher than that of model group (P<0.05).And there is no significant difference in the levels of HOMA-βbetween TangKe decoction of compound Chinese herbs group and rosi- glitazone group (P>0.05).2. Morphological observation of islets:Islets of model group rats have atrophic shapes,rough rims,irregular margins,less cells and gaps,and empty bubbles can be found.After the treatment,TangKe decoction of compound Chinese herbs group and rosiglitazone group have more number cells in dense distribution,and the condition of rosiglitazone group is better than Chinese medicine group.3. Positive expression of apoptosis of islet cells: After the treatment,model group show the higher positive expression of apoptosis of isletβcells significantly than normal control group (P<0.01);the positive expression of apoptosis of islet cells of TangKe decoction of compound Chinese herbs group and rosiglitazone group are significantly weaker than model group (P<0.05),but rosiglitazone group shows a weaker positive expression than TangKe decoction of compound Chinese herbs group (P<0.05).4. Positive expression of Bcl-2 and Bax in isletβcells: After the treatment, model group with Bcl-2 express decreased, Bax express strengthened,and Bcl-2/Bax ratio is low (P<0.05);while TangKe decoction of compound Chinese herbs group and rosiglitazone group compared with Bcl-2 expression increased in model group, and Bax expression reduced,Bcl-2/Bax ratio increased,there are significant difference (P<0.05); but there is no between TangKe decoction of compound Chinese herbs group and rosiglitazone group in islet cell apoptosis positive expression (P>0.05).Conclusion:1. TangKe decoction of compound Chinese herbs group can improve the function ofβcells of islet in deep extent.2. TangKe decoction of compound Chinese herbs group can increase the number of isletβcells significantly.3. TangKe decoction of compound Chinese herbs group can make Bcl-2 protein expression increase, Bax protein expression decline, raise Bcl-2/Bax ratio ,and significantly inhibit islet cell apoptosis.