| Microwave has been used more and more many fields and it's biological action been investigated. Eye is one of the main target organ of microwave. Microwave has effects on visual system primarily involving cornea, lens and retina. It can induce opacity of lens, degeneration and necrosis of retinal neurons, thus effect the visual functions. Most studies concentrated on the damage of lens, but there are fewer reports on the damage of retina. This experiment was designed to observe the damage on cultured human retinal pigment epithelial cells by 2450MHz microwave and the protective effects of taurine on this damage and inquire into the relations between microwave radiation and apoptosis has analyzed. Methods:K The human retinal pigment epithelial cells were cultured in vitro. We observed the growth status of the cells and the effect of microwave radiation on cell proliferation and cell morphologic. According to microwave radiation intensity, retina pigment epithelial cells were divided into control group, 10mW/cm2Xlh group, 20 mW/cm2 X lh group and 30 mW/cm2Xlh. The distance from the microwave source to cells was 16cm. After radiation, cell grow curves were drawn for every group in order to understand the effect of microwave on the cell proliferation and the distribution of cell cycle detection was taken to the cells on flowcytometry. We observed the cells under microscope.2^ The logarithm period cells were radiated by different microwave density (OmW/cm2, 10mW/cm2, 20mW/cm2, 30mW/cm2) for lh, at the same time, the temperature was measured by WDD-1 type thermometer. All cells were collected?after radiation, the following index were measured. Firstly, activity of intracellular SOD and GSH-Px and the content of MDA were detected. Secondly, cells were exposed to trypanblau staining liquid to determine the survival rate. Thirdly, DNA stands breaking was detected by gel electrophoresis.3 -, The cells of protective group had been culture in the medium that contain taurine 5, 10, 20mmol/L separately for 24h before being radiated by 30mW/cm2 microwave. The indexes were measured to observe the protective of taurine. Result:1 ^ The proliferation of the cells radiated are much slower than the control. The morphologic of the 10mW/cm2 group cells did not change dramatically, but some of the cells of 20mW/cm2 and 30mW/cm2 died.2> After the cells were exposed to microwave, the activity of their intracellular SOD and GSH-Px decreased, the content of MDA increased significantly. The survival rate of cells of 10mW/cm2 group didn't changed dramatically, but that of 20mW/cm2 and 30mW/cm2 were decreased compared to the control. DNA ladders occurred in 20mW/cm2 group and 30mW/cm2 group gel electrophoresis. Conclusion:1 % 2450MHz microwave could inhibit the proliferation of human retinal pigment epithelial cells.2^ After exposed to 10mW/cm2 microwave radiation for Ih, human retinal pigment epithelial sells in vitro could be damaged in lipid peroxidation. The extent of the damage improved with the increase of microwave intensity.3 - 2450MHz microwave could induce apoptosis of human retinal pigment epithelial cells in vitro. The cause maybe the following things: (1) microwave could induce intracellular lipid peroxidation, (2) microwave could induce the?damage of DNA, thus induce apoptosis.4> Taurine may act as antioxidation, protect damaged by microwave on the cells. So it could inhibit apoptosis of human retinal pigment epithelial cells induced by 2450MHz microwave. |
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