Human Papillomavirus Infection And Immune Response Studies

Object:To study the immune state of the patients with condylomata acuminata (CA) who were infected by human papiflomavirus(HPV), in order to obtain a clue as to the immunological mechanisms possibiy relevant for wart regression or recurrent. METHOD: (1)Choice of patients: 158 patients with condylomata acuminata had been examined in the study. After those patients were elected by the clinical doctors of the department of dermatology in the first or second affiliated hospital of J!ANXI medical collage and the dermatologists of the affiliated hospital of JIANXI Chinese traditional medical collage and the dermatologists of the JIANXI health center hospital for women and children, the patients were examined to determine positive by HPV6.11 PCR. 20 patients were randomly selected to pathological examination, and confirmed that all of the patients were patients with condylomata acuminata. (2)Dividing groups: Patients were affected CA patients, and 30 healthy persons were selected as control group. According to the state after treatment, the patients were divided into two groups: one group was recurrent group and the other one was no-recurrent group. (3)lmmunity index and method: 0 Cellular immunity: We had examined the 77 CA patients, 31 recurrent patients and 30 no-recurrent patients?lymphocyte subpopulations by APAAP, moreover 30 control subjects were examined also.瓾umoral immunity : We examined the 34 CA patients?se~c immunoglobulin concentration (lgA, lgG, gM), complement factors (03, 04) by automated chemistry analyzer machine. ﹍mmunity molecule: Sandwich enzyme-linked immunosorbent assay (ELISA) was carried to detected the levels of IL-2, IFN-r in the serum of 30 of 158 CA patients and 30 control subjects. In addition, we examined the serum solube intercellular adhere molecule (5ICAM-1) by ELISA. Of 85 CA patients, 31 recurrent patients, 30 no-recurrent patients and 30 healthy control subjects. RESULT: (1) Cellular immunity: The results reveal alterations in the T lymphocyte subpopulations, with a significant decrease in the CD3~T lymphocyte percentage (56.13?.88%), the CD4~T lymphocyte percentage (34.89?.18%) and CD4~/CD8~ ratio (1.2?.11%) and a significant increase in the CD8~ T lymphocyte percentage (28.91?.96%) of the CA patients compared with the healthy control group(P<0.01). The no-recurrent group compared with the recurrent group showed a significant increase in the CD3~ T lymphocyte percentage (64.19?0.54%), the CD4~ T lymphocyte percentage (40.68?.66%) and CD4~/CD8~ ratio (1 .63?.15) and a significant decrease in the CD8~ T lymphocyte percentage (25.35?.53%, P<0.01). T lymphocyte subpopulations of the no-recurrent grouprestored. (2) Humoral immunity : The CA patients compared with the healthy control group showed an increase-3-in the serum gA, 1gM, lgG (1.64 ?0.36 gIl ~ 1.24 ?0.32 g/I~ 8.67 ?0.86 gIl) C3 and C4(1 .45 ?0.27 gII~ 0.297 ?.43 g/l), however only lgG have a significant increase(P<0.05). (3) Immunity molecule: There is a significant decrease in the serum lL-2 (17.75?.52pg/ml, P0.01 )and IFN-y (27.89?.lpg/ml,P<0.05) and a significant increase in the sICAM-1(285.23?.O6ng/ml,P<0.01)of CA patients compared with the control subjects. The no-recurrent group compared with the recurrent group showed a significant descend in the serum sICAM-1 (178. 17 ?37. 43ng/ml, P<0.01) and recover to normal. Multivariate stepwise regression analysis showed that the size of condylomata acuminata and the state of outcome are two of the important factors influencing the serum sICAM-1 changes. CONCLUSION: The infection of HPV effects on the cellular immunity and induces a decrease of the function of cellular immunity, which can restore after recover. Cellular immunity plays a major part in controlling anti-infection of HPV in patients. In addition, the infection of HPV has affected on humoral immunity, and has not affected on complement. The infection of HPV induces a decrease of serum IL-2 and IFN- i ,thus induces a decrease of the function of cellular immunity. So it is c...