Lipid Induced Insulin Resistance On Glucose And Lipid Metabolism

Objective To establish an extended hyperinsulinemic euglycemic clamp technique in conscious rats, and to evaluate the insulin sensitivity and insulin-mediated glucose metabolism in vivo.Methords An extended hyperinsulinemic euglycemic clamp technique with 3-3H labelled glucose as a tracer was applied in conscious rats to investigate the time course of plasma free fatty acids(FFA), insulin levels and glucose turnover during the insulin clamp.Results When insulin was infused at a rate of 4.8mU/kg.min,hepatic glucose production(HGP) was completely inhibited, and the concentration of FFA was remarkably decreased while insulin-mediated glucose disappearance rates (GRd) was significantly increased compared with basal values. The average coefficients of variation in blood glucose levels, glucose infusion rates(GIR)and glucose disappearance rate(GRd) were 2.01%.4.5% and 8.56% respectively. The errors of replication in GIR and GRd were 1.4% and 1.9% respectively.Conclusion Extended hyperinsulinemic euglycemic clamp technique with 3-3H labelled glucose as a tracer in the conscious rats were performed with good precision and credibility. During the steady-state of exogenous insulin and glucose metabolism, the insulin-mediated glucose disappearance rate(GRd) was significantly increased while the hepatic glucose production(HGP) was completely inhibited,and the lipolysis was remakablely decreased.