Qing Kai Ling Effective Components Of Cerebral Ischemic Injury In Neuronal Protection Mechanism

Headstrokes by thrombus blocking cerebral blood supply has become the third death reason andthe first reason of disabling.Thrombolysis and neural protection are two major tactics.Thrombolysis isa clinically major method at present.But it is restricted by time-window of treatment (usually within 3hours) and there are many kind of side-effects.More and more evidences indicate that there is anactive neuronal survial process besides programmed cell death after cerebrals damage. These twokinds of opposite courses are adjusted and controlled by the gene. In neuron survival mechanism,oncogene Bcl-2 by often researched plays an important role in refraining apoptosis .However, thereare also some molecular mechanisms participating in neuronal survival mechanism ,such asneurotrophic factor ,neural protective factor (TGF-β and HSP70) .This study mainly focus onresearch of the neuronal protective mechanismfor the cerebral ischemia . Pathophysiological state of cerebral ischemia is one multi-link,multifactor,many paths cascadereaction promoted by enzymes .Western medicine is intervened among them simply by a certain linkor the treatment with single way is verified that curative effect is very little clinically.TraditionalChinese medicine has characteristic of the organic conception, it also possesses the characteristics ofmedicine made of two or more ingredients again. From studying ,it acts on many targets andintervenes pathological routes of course of cerebral lacking blood damage.It has some certainfeasibility.To seek the effective measures of preventing and treating jointly cerebral ischemia fromtraditional Chinese medicine is the subject of people paying close attention to. At present traditional Chinese medicine protecting pharmaceuticals concentrate on invigoratingblood circulation, having more ideas of waking up one's cerebral,benefiting liver and kidney, reducingphlegm and opening luo .It worths further discussing whether to form the medicine made of two ormore ingredients with curative effect of traditional Chinese medicine.To utilize Chinese medicine andgene engineering and effective biochemical index combine by dialectical theories will solve withclinically curative problem that can be believed experiment of animal as soon as possible.Effectivecomponents of QINGKAILING (清开灵)are mainly baikal skullcap root(黄芩苷), cape jasminefruit(栀子苷), cholic acid(胆酸),nacre(珍珠母).They detoxify and open LUO(络),invigorateblood circulation silt, have one's ideas straightened out and wake up one'sbrain efficiently.To studythis medicine's influences on neurotrophic factor and neural protective factor( TGF-β and HSP70)of cerebral ischemia, it attempts to testify curative effects of cerebral ischemia and expound theprotecting mechanism by QINGKAILING's effective component parts.It will offer modern scientificevidences for traditional Chinese medicine treating cerebral ischemia.Method and Results This experiment consults Longa's method and succeeds in duplicating cerebral ischemia modelof rat. Having succeeded in duplicating cerebral ischemia model, rats are divided into model group,given medicine group and the false surgical group according to two periods of time .The given-4- 请开灵有效组分对脑缺血损伤神经元的保护机制研究medicine group includes baikal skullcap root,cape jasmine fruit,cholic acid,nacre and fourcompatibility groups.According to lacking for blood ,there are 12 hours and 24 hours in two periodsof time. Besides false surgical group, the other groups are given one time of medicine before makingmodel one hour.Drug therapy groups are injected the corresponding medicine through abdominalcavity respectively.When needed to give the medicine for the second time, it is 4 hours with theinterval of given medicine for the first time; When needed to give the medicine for the third time, it is12 hours with the interval of given medicine for the second time.All indexes are measure...