Animal Experimental Wiry Teaching Model And Its Evaluation

1 Objective The experiment copied the wiry pulse by hurting rabbits and internennous drop infusing noradrenalin in rabbits and dogs,using NX-3 multifunctional sphygmograph to note the change of the sphygmogram, analyzing the time domain parameters,and classifying them attributively. It is a benefiting research work for teaching Chinese Traditional Medicine pulse diagnosis, although for applying Chinese Traditional Medicine indication to developing new Chinese drug. 2 Methods 2.1 The experiment about changing electricic pressure values into the pulse-taking pressure values of making pressure standars of sphygmograph. Putting 1g,2g,5g,10g,20g,50g,100g,140g weights on the black1 probe and the black2 probe of NX-3 multifunctional sphygmograph ,putting 1g,2g,5g,10g,20g,50g weights on the purple probe of NX-3 multifunctional sphygmograph. Then we noted the the different pressure numerical value when putting none and different weights on the probes to prove the liner relationship between them. 2.2 Setting up wiry pulse model in achy rabbits We operated on the non-drugged state rabbits, exposure the carotid. After two experienced doctors of Traditional Chinese Medicine who knew nothing affirmed the pulse is wiry pulse, we used sphygmograph to note the sphygmogram of carotid. Then after the rabbits breathed calmly, two experienced doctors of Traditional Chinese Medicine who knew nothing affirmed the pulse was the normal pulse ,we noted the the sphygmogram of carotid under the same pulse-taking pressure. After classifying the parameters in the sphygmogram of carotid, we analyzed them by time domain. 2.3 Setting up Wiry pulse model by internennous drop infusing noradrenalin in rabbits and dogs We operated on the rabbits and the dogs, exposured the rabbits'carotid and the dogs' carotid and brachial artery. Then two experienced doctors of Traditional Chinese Medicine who knew nothing affirmed the pulse is the normal pulse ,we used sphygmograph to noted the sphygmogram of carotid and brachial artery and femoral artery. And then we internennous drop infused noradrenalin in rabbits and dogs ,two doctors affirmed the pulse was wiry pulse, we noted the sphygmogram under the same pulse-taking pressure. After classifying the parameters in the sphy1gmogram, we analyzed them by time domain. 3 Results 3.1 The R value of the balck1 probe of NX-3 multifunctional sphygmograph between the weights and electricity pressure value is 0.9993; The R value of the black2 probe of NX-3 multifunctional sphygmograph between the weights and electricity pressure value is 0.9997; The R value of the purple probe of NX-3 multifunctional sphygmograph between the weights and electricity pressure value is 0.9999. They all have good liner relationship. 3.2 The position used for teaching pulse diagnosis 3.2.1 The rabbits The femoral artery pulses where the place is beyond 9cm of the knee joint 3.2.2 The dogs The femoral artery pulses where the artery and femoral cross. 3.3 The feeling of the pulse when the doctor touch the femoral artery 3.3.1 The rabbit ①The normal pulse: when you touched is softly, you could feel the pulse. when you touch it in normal strength, you could feel it pulse strongly. When you touched in the most strength, it pulse infirmly. The rate of the pulse was 17-18 per human's breath.②The wiry pulse: The rate of the pulse was 14-15 per human's breath. the pulse position was shallow; the pulse shape was longer; the pulse tension was higher. It was like touching the string. The wiry pulse model of achy rabbits last for 30.07±4.06 minutes. The wiry pulse model about noradrenalin was felt by doctors during internennous drop infusing noradrenalin. 3.3.2 The dog ①The normal pulse: The rate of the pulse was 8-9 human's breath. when you touched it in normal strength, you could feel it pulse strongly. When you touched in the most strength, it pulse infirmly. ② Whole feeling likes the rabbit's feeling, but the pulse was stronger than then rabbits' even than the beings', and the pulse shape was longer. The rate of th...