Angelica-inducing Yupingfengsan The Th1/th2 Cytokines In Patients With Chronic Urticaria In Peripheral Blood Mononuclear Cells Secrete Experimental Research

Objective: chronic urticaria (CU) is the skin and mucous membrane disease that is caused by multiple factors.the key feature of CU are edema wheal stoking for many time, feeling pruritus in self-consciousness ,progress prolonging above six weeks,even causing Throat mucous membrane that endanger life, because of complicated etiological factors and longer progress ,many time breaking out,difficult prevention and bad treatment effect of routine Antihistamine ,study of the pathogenesis, prevention and cure on chronic urticaria has become important topic in medical science kingdom.Investigation of modern medicine detect: function diseqilibrium of T Helper cell sudgroup(ThlandTh2)and chronic urticaria has close correlation. Secretory cytokine level of CU patien PBMC decreases, but lelve of Th2 cytokine steps up.After treatment Th1 cytokine and Th2 cytokine trends to equilibration,therefoer chronic urticaria is a skin immune inflammatory reaction that Th2 cells hold main status.Traditional medicine reserves effective prescriptions to therapy chronic urticaria,but since many years the investigations to aim directly at it has only limited to clinical observation and profound experiment invesgations are absenct.The experiment used classical prescriptions from traditional medicine that were used to therapy chronic urticaria—Dang Gui Yin Zi and Yu Ping Feng San —and the experiment investgated the effect of secretory Thl and Th2 cytokines from PBMC of chronic urticaria patiens and tried to find out tentative mechanism, and the scientific theoretics foundations were going to provided to the classical prescriptions—Dang Gui Yin Zi and Yu Ping Feng San—to therapy chronic urticaria.Method:1 Selective standard of idiopathic chronic urticaria patiens1.1 The surface of Skin and mucous membrance occurred recurrent wheals and the duration was not beyond twenty-four hours.1.2 The incidence could not be under two times weekly.1.3 The progress was over six weeks.1.4 The antihistamine drug was not used in one week and the steroid hormone and the immunosuppression drug were not used in one month.1.5 The physical uricaria was excluding because of the coldness and the pressure and so on.1.6 The autoimmunity diseases was excluding.2 Monocytic separated technique in venous blood:there were thirty-one patiens of chronic urticaria and fresh venous blood was going to be drawn from every patien.In order to obtan Lymphocytes and Monocyts the Ficoll iso-sedimentation separation was used and the cell suspension was produced with RPMI1640 and the cellular tele-concentration was 1×105/ml.3 Detecting method of cellular vital force:the trypan blue exclusion test was selected and after staining with 0.4% trypan blue counted vital cells could be above 95% in three minutes.4 Routine obseration of cultured cells4.1The changes of form and tiny formation:the volum of lymphoblasts largened and the nuclei were transparent and the ball agglutination phenomenon occurred.4.2 Observation of culture medium:the normal fresh culture medium containing phenolsulfonphthalein was peachblow and as soon as it became primary colors and the possibility of bacteria contamination needed be attented.5 The cultivation of pro-generation cells: the cells were coltured in vitro with RPMI1640 culture medium in the 96 cell culture plates and had been hatched for forty-eight hours in the carbon dioxid incubator on the condition of 37℃,and then had been centrifugated by means of 2000r/min for 20min,at last the superior-schedul liquor were collected and were preserved on the condition of-20℃.6 The pretest about drug susceptibility invitro:the MTT colorimetric assay was adopted.The extract of Dang Gui Yin Zi and Yu Ping Feng San were used and acted at PBMC of chronic urticaria alonely or associating with PHA in vitro,and then the cytotoxic action was detected by means of OD numeric value.7 Dividing into groups of experiment:the experiment were divided into the blank-contrast group and the experiment-medication group,and every group were all designed three pores,the pores of...