Topical Gynostemma Light Aging Model Mice Skin Tissue Sod Activity And Mda, Hyp In Content Affect The Experimental Study

Objective : To investigate G.Pentaphyllum's influence with ultraviolet A (UVA) and ultraviolet B (UVB) on the content of hydroxyproline(HYP)and Malondialdehyde(MDA)in the skin of hairless photo ageing mice as well as the activity of Super oxide Dismutase (SOD) in their skin, and to investigate G. Pentaphyllum's effect on the structure of the hireless photoageing mice.Methods: Healthy KM hairless mice were divided into seven groups randomly and gavages respectively, that is negative Control Group ( A ) ,ModelGroup ( B ) , Model+Low-G. Pentaphyllum group ( C ) , Model+Medium-G. Pentaphyllum Group ( D ) ,Model+High-G. Pentaphyllum Group (E) .The mice were irradiated to simulate skin photoageing by long-term ultraviolet irradiation, at the same time, Model+excipient is maquillage brand snow, group C, and D, E were given with G. Pentaphyllum and Model+excipient. Staining skin with HE, Under 40 times light microscope, the structure of dermal were observed. While the changes of the contents of dermal hydroxyproline and Malondialdehyde, as well as the activity of Superoxide Dismutase were measured by biochemical test method in each group of the experiment.Results: In model group, the activity of SOD and the content of HYP of dermal was decreased, while the cortent of the MDA was increased seriously (P<0.05) . The skin biopsies appear photoageing look. The contents of SOD and MDA in dermal in group C, D, E had no difference with that in group A, the contents of HYP in dermal in group C, E had no difference with that in group A also. Compared with the group B, the content of HYP was increased, while the content of MDA was reduced in group C, D, E (P<0.05, P<0.01) , the activity of SOD in dermal in group C, D was increased (P<0.05) .Conclusions: G. Pentaphyllum has the protective effect on the skin of the photoageing hairless mice with ultraviolet irradiation. The mechanism may be that on one hand, G. Pentaphyllum could eliminate free radicals itself; on the other hand, by increasing the activity of SOD, it could inhibit the oxidative damages of ultraviolet and skin photoageing.