Hif-1 Alpha And Discussion On The Role Of VEGF In Patients With Cerebral Ischemic Tolerance

Object:To investigate the expression of HIF-1α in the model of ischemic tolerance induced by focal cerebral ischemia preconditioning in rat and protective effects of focal ischemical preconditioning on middle cerebral artery occlusion(MCAO) in rats. Methods:A totle of 112 healthy Wistar rats were divided into 4 groups randomly, the control group(28),the ischemic preconditioning(PC)group(28),MCAO group (28)and PC+ MCAO group(28). ischemia tolerance(IT) models were established by occluding unilateral MCAO of the rats with the suture method. The expression of HIF-1α mRNA was analyzed by RT-PCR,the expression of HIF-1a protein was analyzed by Western .Infarct size, post-infarction neurological deicits and brain water were measured. The histological damage was evaluated by HE staining and electron microscope. Results:RT-PCR :The contral group and PC group exprect low HIF-1 a mRNA , D/D0 :0.208±01270 and 2165±0.127, there was no significance between groups;the expression of HIF- 1αmRNA was greatly enhanced in PC+MCAO group and in MCAO group,D/D0 :0.75±0.023 and 0.30±0.027, p<0.05,there was significance between groups. Western:the contral group and PC group did not exprect HIF-1 αprotein;The protein of HIF-1 a was 26±0.032 in MCAO group and 0.50±0.012 in PC+MCAO group , p<0.05,there was significance between groups.produced no brain injury and no neurologic impairment and infarct was identified in the PC group. Compared with MCAO group,no neurologic deficit scores, infarct size and brain water conttent were significantly reduced in PC+MCAO group,there was significance between groups. Conclusions : PC provided brain protection to subsequent MCAO.That PC sigificantly improved HIF-1αafter MCAO suggest PC might induced IT in brain so that reduced brain injury and proteins expressin. The present experiment shows IT is associated with changes in HIF-1α expression.Object: To investigate the expression of VEGF in the model of schemic tolerance induced by focal cerebral ischemia preconditioning in rat and protective effects of focal ischemical preconditioning on middle cerebral artery occlusion in rats. Methods: A totle of 40 healthy Wistar rats were divided into 4 groups randomly, the control group(10),PC group(10),MCAO group (10)and PC+ MCAO group(10).IT models were established by occluding unilateral MCA of the rats with the suture method. The expression of VEGF was analyzed by RT-PCR and Western . Results:RT-PCR :PC and Control did exprect VEGF mRNA ,D/D0 :0.29±0.093 and 0.28±0.021,there was no significance between groups;the expression of VEGF mRNA was greatly enhanced in PC+MCAO group and in MCAO group , D/D0 : 0.74±0.031 and 0.59±0.022,p<0.05,there was significance between groups. Western:the contral group and PC group did exprect VEGF protein ,the protein of VEGF was 0.21±0.032 and 0.22±0.010, there was no significance between groups;The protein of VEGF was 0.27±0.012 and 0.41±0.012,p<0.05,there was significance between groups. Conclusions:That PC sigificantly improved VEGF after MCAO suggest PC might induced IT in brain so that reduced brain injury and proteins expressin. The present experiment shows that IT is associated with changes in HIF-1a and VEGF expression.Further understanding the mechanism of endogenous neuroprotection is needed for finding a novel therapeutic strategies for acute ischemic cerebral infarction management.