.ag43/fc¦Å3 Chimeric Bacterial Surface Antigen Protein Vaccine In Mice With Asthma Preventive Effect

Objective: To explore whether Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination could induce an autoimmune response against self-IgEFcε3 structural domain, break of the immune tolerance against mouse IgEFcε3 structural domain, produce autoantibodies against self-IgEFcε3 structural domain and eventually play a role in treatment of of asthma in murine models.Methods: Ag43/Fcε3 chimeric bacterium surface antigen protein was used as a vaccine to validate whether or not it had effects on prevention and cure of asthma in murine models by observating influence on mouse symptoms, inflammatory cell infiltration, airway hyperreactivity(AHR), levels of serum total IgE in peripheral blood, IL-4 and IFN-γ in lymph node (LN) cells. Autoantibodies against self-IgEFcε3 structural domain were detected by ELISA, Western blot analysis and ELISPOT assay. The effects on prevention and cure of asthma with the autoantibodies were certified by the immunoglobulins purified from the serum of the immunized mice.Results: Asthma therapy with Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination was effective at both preventive and therapeutic antiasthma immunity in murine asthma models. Murine IgE specific autoantibodies in sera of mice immunized with Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination could be found by Western blotting analysis and enzyme-linked immunospot assay. Anti-IgEFcε3 structural domain antibody-producing B cells in spleens could also be found by ELISPOT assay. The major antibody subclasses induced by the Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination immunization were IgG1 and IgG2b. The effects on prevention and cure of asthma with purified immunoglobulins were similar to those of active immunization with Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination.Conclusion: Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination had effects on prevention and cure of asthma in murine asthma models. Ag43/Fcε3 chimeric bacterium surface antigen protein vaccination could induce an autoimmune response directed against self-IgEFcε3 structural domain, break of the immune tolerance against mouse IgEFcε3 structural domain. Eeffects on prevention and cure of asthma were resulted from producing anti-IgEFcε3 structural domain antibody. Active vaccination against IgE as therapeutic approach for the treatment of asthma has a potential future for clinical application and is worth of being further studied.