| 1 Experimental study of rats with diabetic nephropathyObjective To establish model of diabetic nephropathic rats,and to observe their general condition, blood pressure, blood sugar, urinary protein/24h, renal function, pathological changes of renal tissue.Methods 32 rats were randomly divided into control group (control group), diabetic nephropathic model group (model group), which had two sub-groups according to different time (4w, 12w), every group had 8 rats. We established the rat model of diabetic nephropathy induced by streptozotocin (STZ), observed the blood sugar, average arterial pressure, urinary protein/24h, serum creatinine (Scr), blood urea nitrogen (BUN), and counted endogenous creatinine clearance rate (Ccr); we also observed the renal pathological changes by optics microscope and electronic microscope.Results The blood sugar of model group rats which were injected STZ was higher than that of control group in 2w, 4w, 6w, 8w, 12w. Average arterial pressure, urinary protein/24h, SCr, BUN of model group rats were higher distinctly than those of control group, while Ccr of model group rats was lower than that of control group,(respectively,P<0.05).Conclusion We established successfully the rat model of diabetic nephropathy, which was the basis of our futher research of drug intervention.2 Experimental study on effect of Bailin capsule on diabetic nephropathy in ratsObjective To observe the effect of Bailin capsule on blood sugar, urinary protein/24h,renal function,and pathological changes of renal tissue, and the expression of transforming growth factor-β1(TGF-β1) in rats with diabetic nephropathy.Methods 24 rats were randomly grouped into control, model and treatment,8 rats for each group. Two days after models' establishment,Bailin capsule was administrated (2.5g/kg) by a stomach tube in treatment group, and same amount of water was fed in model and control group. Then we detected the blood sugar, urinary protein/24h, Scr, BUN, calculated Ccr, and used optics microscope to observe the the renal pathological changes. The expressions of TGF-β1 in renal tissue were investigated by immuno-histochemiccl method.Results After 12w, the blood sugar, urinary protein/24h, Scr and BUN in model group were significantly higher compared with control group(respectively, P<0.05). However, Ccr in model group was lower than that of control group, and there was obvious renal tissue damage (respectively, P<0.05). After treatment of Bailin capsule, urinary protein/24h, Scr,... |
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