| In this year,due to the using of extended–spectrum-lactamase antibiotics,the mechanism of bacteria s resistance is varying.Bacteria can produce extended-spectrum-β-lactamase by plasmid,it can hydrolyzeβ-lactam annulus ofβ-lactamase antibiotics,making it lose its ability of sterilization.Its main speciality is that it can hydrolyaze penicillins,cephalosporins and monobactams antibiotics,but it can not hydrolyaze cepharmycins and carbopenems antibiotics.Because of expanding resistance,transfering resistance by plasmid,the fast increasing rate and finding out new genotypes,the resistance has become a severe commonality problem which we should attach importance to.ESBLs have been prevail in the world. In 1997 and 1998, the research in Europe, America and Western Pacific Ocean has indicated that the number of pneumococcus which can produce extended-spectrum-β-lactamase is 45.4% in Latin America, 24.6% in Western Pacific Ocean, only 4.9% in Canada[24]. A research in Italy indicate that the rates of producing extended-spectrum-β-lactamase in pneumococcus and E.coli are 37.1% and 10.8%[25].The rate of extended-spectrum-β-lactamase in China is increasing in these years. Chen has reported that in 1994 the rates of E.coli which can produce extended-spectrum-β-lactamase is 10%, pneumococcus is 12%, in 2000 the rate reach to 25% and 30%, but in 2001 it come up to 35.3% and 32.7%[26].The rate of resistance bacteria in 1356 bacteria which are separated from patients is increasing year by year in the dongfang hospital, E.coli, Pseudomonas aeruginosa, Staphylococcus aureas, pneumococcus and Acinetobacter are most familiar ones. The number of E.coli and which can produce extendedβ- lactamase is rising in these years, the percent of E.coli is 47%(306),and the percent of pneumococcus is 25%(119)[7]. The appearance and diffusion of this resistant bacteria bring a new difficult problem to clinic treatment.Objective: To validate the growth inhibition function of herb to resistant bacteria by studying Shuanghuanglian growth inhibition function to extended-spectrumβ- lactamase E.coli outside body. And to probe into the effection of growth inhibition function by using Western medicine and Chinese traditional medicine. To lay a solid foundation to the growth inhibition function inside body, the mechanism and the clinic study.Methods: To identify resistant bacteria and filter model by using VITEK-32, to validate the shady bacteria by means of the method of slip diffusion which is recommended by CLSI/NCCLS, to study the growth inhibition function of shuanghuanglian and shuanghuanglian associate with shupushen to extended-spectrumβ- lactamase E.coli by using the method of broth dilution which is recommended by CLSI/NCCLS.Results: The Minimal inhibitory concentration(MIC) of shuanghuanglian to extended-spectrumβ- lactamase E.coli is 70mg/ml. The Minimal inhibitory concentration(MIC) of shupushen to extended spectrumβ- lactamase E.coli is 31.25ug/ml.If the concentration of shuanghuanglian reach to 60mg/ml,after 6 hours adding shupushen make the concentration of shupushen be 15.625ug/ml that extended-spectrumβ- lactamase E.coli can not growth. Compared with the group of using shuanghuanglian alone ,the concentration of shuanghuanglian has reduced 14.3%. Compared with the group of using shupushen alone ,the concentration of shupushen has reduced 50%.Conclusion: The growth inhibition function of shuanghuanglian to extended-spectrumβ- lactamase E.coli is powerful. When shuanghuanglian associate with shupushen(space a certain distance ) function to extended-spectrumβ- lactamase E.coli outside body, it can make the effect much greater than before. |
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