Gentian Total Glucosides Of New Drug And Its Preparations Of The Aerial Parts Of Gentiana High-speed Countercurrent Chromatography

Longdan total glycosides from the roots of Qinjiao is iridoids, registered drug by Xi'an C.P. Pharmaceutical Co., Ltd .Then this company developed it as a new drug .The brand name is "Jin ti na".Qinjiao is a rare medicinal substance, so look for the substitution of Qinjiao and expand the using parts of Qinjiao is urgently to be solved .In deep autumn when qinjiao is harvest,the aerial parts of Qinjiao is abondonned ,this is great waste.So,the research and development of the aerail part of Qinjiao solved the problem of waste and insufficient .The optimum extract method was selected by using of orthogonal experiment ,and the optimum model of resin was selected by comparing the adsorptional ability of different resins to gentiopicroside.Meanwhile by adsorptional and unadsorptional experiments,we determined the technological conditions.After all,we get the extractive coherent the quality criteria of Longdan total glycosides from the roots of Qinjiao . The pharmaco-experiment demonstrated that this extractive from the aerial parts of Qinjiao coherent the Longdan total glycosides from the roots of Qinjiao in pharmacodynamic action.Based on this research, The extractive was prepared into film-coated tablets.In the tablets production process,the problems of absorbing moisture and caking softened were solved.At last ,the quality criterias of film-coated tablet and the aerial part of Qinjiao were researched.High-speed counter-current chromatography(HSCCC) was successfully applied to the preparative separation of gentiopicroside from the crude extract of the aerial part of Gentiana macrophylla Pall using a two-phase solvent system composed of chloroform methanol and water(4:4:2 in volume ratio).The quantity of the sample increased by degree from 50mg to 300mg ,the separated fraction was gentiopicroside as identified by LC-MS,every separation yields gentiopicroside at 94% purity as determined by HPLC.The thesis includes the following four sections :Part one is the research on the technology of extracting longdan total glycosides from aerial part of Qinjiao;.Second part mainly about the research on the preparation technology of Longdan total glycosides Filmlok.;The third part is the quality criteria of arial part of Qinjiao and Longdan total glycosides Filmlok and the illustrations of drafting this two quality eriterias; Part four is mainly about preparative separation of gentiopicroside from aerial part of Gentiana macrophylla Pall by high speed countcurrent chromatography...