Qi Decoction On Proliferation And Apoptosis Of Vascular Smooth Muscle Cells Of The Arterial Injury In Rabbits

Objective: To investigate the effect of Promoting Qi to promote bloodcirculation prescription(PQPC)on smooth muscle cell proliferationand apoptosis in high-fat fed rabbit' s vascular wall after vascularintima was injured.Method: 32 Male New Zezland white rabbits were divided into fourgroups randomly. (1)Normal group; (2)Control group; (3)Chinesetraditional medicine group; (4)western medicine group. Beforeinjured vascular intima which were fed by high-fat diet for 4 weeksexcept normal group, and put the iliac artery endarterectomy ofrabbit into practice from the fifth week. From beginning(firstweek)two kinds of medicine were fed respectively till the end. At thesixthweek, we can take blood from the vein of the edge of rabbit' sear to test lipid with backman' s 700 automatic biochemical enzymemethod, serum totalcholesterol(TC), triglycerides(TG), low desitylipoprotein cholesterol(LDL-C) high desity lipoproteincholesterol(HDL-C)were measured. At the same time, local vascularswere collected for platelet derived growth facor-B(PDGF-B),c-mycand Insulin-like growth fator-1(IGF-1)levels measurement. Under LMand TEM you can get a pathomorphological observation.Result: (1) TC, TG, LDL-C, HDL-C increased significantly in controlgroup compared with the normal group(P＜0.05); TG in western medicinegroup and Chinese traditional medicine is lower compared with thecontrol group(P＜0.05);TC, TG, LDL-C, HDL-C decreased significantlyin Chinese traditional medicine group compared with the westernmedicine group(P＞0.05). (2) PDGF-B, c-myc and IGF-1 increasedsignificantly in control group compared with the normal group(P＜0.05). PDGF-B, c-myc and IGF-1 decreased in Chinese traditionalmedicine group and western medicine group significantly comparedwith the control group(P＜0.05). (3)The pathological changes of bloodvessel ease obviously under the observation of naked eye, LM and EM.Conclusion: (1)The Promoting Qi to promote blood circulationprescription can adjust the blood-fat level of the atherascleracicrabbits. (2)It evidengly restrains the formation of theatherasclerasis of the rabbits which were fed on high fat feedstuffobviously. After vascular intima injured, PDGF-B, c-myc, and IGF-1 increased can promote smooth muscle cell proliferation and restrainit apoptosis. (3)The promoting Qi and circulating Blood can reducethe expression of PDGF, c-myc and IGF-1 after the arterialinjury, which expression of PDGF, c-myc and IGF-1 after the abterialinjury, which means it restrains the proliferation of smooth musclecells on some degree after the injury of afteries.