Using Free Radical Scavenger Edaravone Study Of Free Radicals In The Process Of 1,2 - Dichloroethane (1,2-the Dce) Induced Brain Edema

[Objective]To explore the effect of 1,2-DCE in brain edema process and provide theoretical foundation for the prevention,diagnosis and treatment for acute intoxication of 1,2-DCE.[Method]The main measurements have two parts:in vitro and in vivo.In the first part we established the mouse brain edema model induced by 1,2-DCE and then peritoneal inject edaravone(free radical scavenger)followed treatment of 1,2-DCE.After the tissue section and electron microscopic section were made,we investigated the relationship between SOD,MDA,GSH concentration and brain edema.The second part,we treated cortical neuron with different levels of 1,2-DCE at different time and then the SOD,MDA,GSH activities were detected to discuss the mechanism of brain edema induced by 1,2-DCE.[Result]In the comparison of brain weight between different groups we found that 1,2-DCE treated mouse contains much more water in brain than groups added free radical scavenger.In other groups we found the activity of SOD, GSH rised and MDA dropped with the increasing of scavenger concentration.In cell culture, besides cell morphology diversity we also found the similar activity change in SOD,GSH and MDA.[Conclusion]According to the fact that the addition of edaravone,a kind of free radical scavenger,significantly depressed the oxidative damage induced by 1,2-DCE,free radical plays an important role in the brain edema process induced by the 1,2-DCE.