Heme Oxygenase-1 Expression In The Role Of Endotoxemia In Experimental Cirrhosis

Objective:To investigate the effect of the expressions of HO-1 and iNOS in lipopolysaccharide(LPS)-treated cirrhotic rats from induced oxidation and antioxidant protection systems.Methods:Liver cirrhosis was produced by the administration of thioacetamide for a period of 10 weeks.Forty-two male Wistar rats were randomly divided into LPS tareat normal Group (control group),cirrhotic group(TAA group),AG and LPS taread cirrhotic rats group(AG group),LPS tarted cirrhotic rats group(TAA+LPS group),hemin and LPS tarted cirrhotic rats group(HM group),each group had 8 rats.All rats except of control group weregiven thioacetamideas drinking fresh water.They drinked 0.03%and 0.04%thioacetamide erch for five weeks.Cirrhotic rats in TAA group were injected with 0.9%NaCl.All rats except of TAA group were injected with a 3-mg/kg dose of LPS.HM group were injected with a 40-mg/kg dose of hemin befor 24 hours injected LPS.,AG group ere injected with a 50-mg/kg dose of aminoguanidine after24 hours injected LPS.All ratswere sacrificed 6 h after LPS or salineinjections,rats were weighed,anesthetized byan intraperitoneal injection with 7%chloral hydrate(5ml-kg 1).Blood samples were collected from animals by abdominal aorta puncture. Blood samples was centrifuged at 3,000 rpm for 10 minutes at 4℃and stored at -80℃′Plasma alanine transaminase(ALT) and aspartate transaminase(AST) activities were determined using kits fromSigma.Plasma NO formation was quantified by its oxidation products,nitrite and nitrate.Nitrate was reduced to nitrite by nitrate reductase and total nitrite levels were determined through a color reaction with the Griess reagent.the level of Plasma malon-dialdehyde(MDA) formationwas measured by the thiobarbituric acid method that measuresaldehydes as an end-product of lipid peroxidation.The protein expressions of HO-1 and iNOS were observed by using immunohistochemical technique and assessed through BI-2000 image analysissystem semi-quantitatively.Results:(1) the protein expressions of HO-1:The ILD of the stained HO-1protein in the liver tissues of control group,TAA group,AG groupTAA+LPS group andHM group.were significantly different and increaded by and by(P＜0.05).(2) the protein expressions of iNOS: The ILD of the stained iNOS protein in the liver tissues of control group,TAA group,AG group,TAA+LPS group andHM group.were significantly different and increaded by and by(P＜0.05).(3) ALT,AST,and NO in Plasma of control group,TAA group,AG group,TAA+LPS group andHM group.were significantly different and increaded by and by(P＜0.05). Plasma MDA in control group TAA+LPS group andHM group.were significantly different and increaded by and by;Plasma MDA in.AG group and TAA group were significantly different(P＞0.05).Conclusions:The damagement effect of HO-I induction,at least in part,via the activation of iNOS/NO system,maybe involved the ONOO- induced oxidation pathophysiological processpathophysiological process of LPS-treated cirrhotic rats.The results suggest that the protective effect of AG,a selective inhibitor of iNOS at least in part,via preventing the activation of iNOS/NO system,may be involved preventing the ONOO-induced oxidation pathophysiological process of LPS-treated cirrhotic rats.