Ganoderma To Improve Learning And Memory And Its Mechanism

PartⅠof the 2005 edition "Chinese Pharmacopoeia" containing Ganoderma for the dry.carpohole of Ganoderma lucidum(Leyss,ex Fr.) Karst or Ganoderma sinense Zhao,Xu et Zhang.It has a function of invigorating Qi for tranquilization,relieving cough and asthma.As the research of growing and breeding of Ganoderma is mostly worked by agricultural scientists,it lack of systematic research of this "traditional medicine".Moreover,medical fungus registration system in china has not been perfect, this also result in hybrid varieties of fungus resources,genetic relations unclear.And varieties species have differences in chemical composition and pharmacologic action. So the confusion of varieties Ganoderma species in clinical application restrict it's develop and application.Ganoderma is commonly used by Asian countries as antiaging agent,modern pharmacological research show that Ganoderma has strong abilities of anti-tumor, regulating immune function,treating cardiovascular and cerebrovascular diseases.But the research of it's protective effect of the nerve is very little.In response to these phenomenon,in this paper,Genetic specificity of eight different strains of Ganoderma had been studied preliminarily,screening test of different strains of the fungus in improving the effect on learning and memory had been done and the water maze test and step-though test were carried out to explore the changes of the rats' learning and memory capacities by the selected Ganoderma strains,ZL2.The selected strains,ZL2,had been explored the changes of the rats' learning and memory abilities by maze test and step-though test.Determination of Cholinergic neurotransmitter as a method has been adopted to study it's effect on neurotransmitter.We used an in vitro model system,PC12 cells,to observe the resistive effects of serum and cerebrospinal fluid containing ZL2 on neurotoxicity induced by Beta-amyloid protein.The results showed that:1.There are strong antagonistic relationships and obvious phaenotype genetic specificity of different sources of 8 strains,except ZL3 and ZL4.2.Behavior experimental study show that ZL2 presented evident effects on learning and memory.ZL2 can improve learning and memory capacities of normal animals,aged mice,animals injuried in learning and memory capacities,and have positive effects on learning and memory on different stages. 3.Mechanism explore show that the improving effect on learing and memory of ZL2 has relationship with the increase of content of acetylcholine in cerebrum and the decrescence of acetylcholine esterase enzyme activity.Role in nerve protection,ZL2 promote proliferation of PC12 and resist the neurotoxicity induced by Beta-amyloid proteinIn short,different kinds of Ganoderma strains have genetic specificity and different pharmacological properties.The selected strains of fungus,ZL2,presented promising effects on learning and memory,the mechanism has relationship with the increase of acetylcholine content in cerebrum and the decrescence of acetylcholine esterase enzyme activity,also has relationship with neuroprotective effect.