Kidney Medicine On Experimental Diabetic Ed Rats Neurotransmitter Nos Isoforms Mrna

Objective: Determination of NOS in the ED in diabetic rats and corpus cavernosum neurotransmitter mRNA expression of NOS isoforms, KRH study of ED in diabetic rats corpus cavernosum neurotransmitter NOS mRNA isoforms affected. KRH treatment of diabetic erectile dysfunction in the mechanism. Methods: The rats were divided into seven groups, the normal group (A), STZ group (H), diabetes (group B) and have the erectile dysfunction drug in the treatment of ED group (D), perfusion meeting Renal medicine high-dose group (E), low dose group (F), the WM group (G), and then use Chinese medicine and western medicine perfusion Bushen diabetic erectile dysfunction in rats treated eight weeks after the treatment, drawing large rat corpus cavernosum, corpus cavernosum tissue by RT-PCR kit to the measurement and NOS mRNA expression Trizol extraction. Collecting cells from the total RNA, reverse transcription into cDNA templates for RT-PCR, PCR system for 30 cycle, the reaction system 80 ul. After the reaction, the reaction solution from 10 ul bromide tablet containing 2% agarose gel electrophoresis of 30 minutes (5 V / cm), UV analyzer observed in the 501 bp if occurring at the orange bands for positive samples. PCR products can be -20℃frozen for later analysis using gel imaging systems camera. Using SPSS statistical data, the data that group. Results: diabetic erectile dysfunction (ED) in the corpus cavernosum NOS activity, and nNOS (neuronal NOS) protein expression in normal group, STZ group, diabetes control group decreased significantly. Diabetes and erectile dysfunction group and the normal group, STZ group, the diabetic group compared to the penis around the cavernous nerve iNOS (inducible NOS) protein expression. Erectile dysfunction in diabetic rats group corpus cavernosum iNOS and nNOS ART - polymerase chain reaction (RT-PCR), and STZ group and the normal group, the diabetic group compared with its lower protein content, the lower the level of mRNA. Analysis of data obtained There was a significant difference (P <0.01). Conclusion: KRH can improve diabetic erectile dysfunction group NOS mRNA and protein content of the expression of the diabetic erectile dysfunction treatment of a certain effect.