Skeletal Muscle Satellite Cells On The Protective Effect Of Myocardial Tissue

The adult heart,like other organs,contains a population of precursor or stem cells,which are capable of some degree of endogenous repair when the myocardium is injured.However,it is clear in most cases,that after cardiac injury any demand for repair exceeds the capacity of the heart to respond.Thus,after acute myocardial injury,the damaged myocardium usually progresses through a series of events including inflammation,cardiocyte apoptosis,formation of a noncontracting fibrous scar,alteration in the workload of the surrounding myocardium (compensation) and,if the infarct is large enough,ultimately decompensation and deterioration to congestive heart failure(CHF). Current treatment options for these hearts include medical management, cardiac transplantation,mechanical circulatory assist devices(LVADs), or other experimental techniques(artificial hearts).All the attempts can only support the remaining healthy myocardium and it is not possible to replace the damaged organ.Based on the problem,Cell-based myocardial repair and regeneration initiate a new frontier in the treatment of cardiovascular disease.It provides a new opportunity to treat the loss of cardiomyocytes that occurs after myocardial injury and that results in the series of events leading to heart failure.Cell transplantation is a kind of technique that the stem or progenitor cells are delivered to the site of cardiac injury,and we can restore blood flow and contractility to previously scarred heart.Preclinically,many types of cells have been transplanted into injured myocardium - including fetal cardiomyocytes,skeletal satellite cells(SC),smooth muscle cells, embryonic stem cells and bone-marrow-derived stromal cells.SCs locted at between muscle cell membrane and basement membrane is one kind of cells which can proliferate and differentiate.SCs is activated when the musle is injuried and recover the injury.MI mades the number of the myocardial cells decrease and effect the function of the heart.SCs is better than bone marrow stem cells which can be transplanted to cure the injury.Methods:We can activated SCs by injury the skeletal muscle with cardiotoxin.When the skeletal muscle is digested with collagenaseâ… and Trypsin,SCs were isolated and purified with discontinuous density centigugation(Percoll gradient liquid).After establishing stable culture methods for rats SCs and identification of SCs with myosin monoclonal antibody and Immunofluorescence staining,.After being cultured in the cell culture medium which SCs had lived in,myocardial cells' HSP70 content was detected.Results:After SCs were digested with mixture of collagenaseâ… and Trypsin,isolated and purified with discontinuous density centigugation, SCs purity could be as high as 90%.SCs look like spindles and fused into myotubes.By myosin Immunofiuorescence staining tests, Fluorescence signal was found in the cytoplasm.The myocardial cells containd more HSP 70 which were cultured in the Cell culture medium which SCs had lived in than those in the ordinary cell culture medium.Conclusion:1Chemical injures can activeted SCs2The number of activated SCs is the most after 3 or4 days.3The myocardial cells cultured in the cell culture medium contain more HSP70 than those in the ordinary cell culture medium.